OPEN ACCESS
PEER-REVIEWED |
RESEARCH ARTICLE
DNA barcoding application of garfish (Hemirhampus sp.) in North Maluku Sea
Article Sidebar
How to Cite
The article can trace at:
Main Article Content
Abstract
The purpose of this study was to identify the species of garfish in the waters of north Maluku by applying DNA bar-coding techniques. The method used in the study was the primary design of DNA extraction and isolation, and DNA amplification through polymerase chain reaction. The results showed that Gen Cyt b analysis amplified ± 350 bp amplicon and for the alignment analysis showed that the amplification sample sequence had similarity with the Cyt b gene sequence of Hemiramphus balao with value of 97%. Sequence analysis showed that the six specimens had very low genetic distances. It can be concluded that the isolate samples of garfish have a very close kinship.
Abstrak
Penelitian ini bertujuan untuk mengidentifikasi spesies ikan julung-julung (Hemirhampus sp.) di perairan Maluku Utara melalui aplikasi DNA barcoding. Metode yang digunakan dalam penelitian adalah desain primer, ekstraksi dan isolasi DNA serta amplifikasi DNA dengan PCR. Hasil penelitian menunjukkan bahwa analisis gen Cyt b yang diamplifikasi diperoleh amplikon sebesar 350 bp, dan analisis penyejajaran menunjukkan bahwa sekuen sampel amplikon memiliki similaritas dengan sekuen gen Cyt b isolat Hemiramphus balao sebesar 97%. Analisis lanjut me-nunjukkan bahwa keenam sampel memiliki jarak genetik yang tergolong sangat rendah. Dapat disimpulkan bahwa sampel isolat ikan julung-julung dalam penelitian ini masih memiliki hubungan kekerabatan yang sangat dekat.
Downloads article
Article Details
Copyright (c) 2019 Jurnal Iktiologi Indonesia |
|
|
References
Akbar N, Zamani NP, Madduppa HH. 2014. Ge-netic diversity of yellowfin tuna (Thun-nus albacares) from two populations in the Moluccas Sea, Indonesia. Depik, 3(1): 65-73.
Akbar N, Aris N, Irfan M, Tahir I, Baksir A, Surahman, Madduppa HH, dan Kotta R. 2018a. Filogenetik ikan tuna (Thunnus spp.) di Perairan Maluku Utara, Indone-sia. Jurnal Iktiologi Indonesia, 18(1): 1-11.
Akbar N, Aris M. 2018b. Genetic population structure of yellowfin tuna (Thunnus albacares) as based data of fish conser-vation in North Mallucas sea. Omni Akuatika, 14(3): 75-85.
Akbar N, Irfan M, Aris M. 2018c. Population genetics and phylogeography of bigeye tuna in Moluccas Seas, Indonesia. Ilmu Kelautan, 23(4): 145-155.
Aris M, Akbar N, Labenua R. 2017.Genetic and phylogenetic variations of yellowfin tuna (Thunnus albacares) as a basis for sus-tainable fishery resources management in North Moluccas. International Journal of Pharma Bio Science, 8(4): 419-426.
Asiah N, Sukendi, Junianto, Yustiati A, Windar-ti. 2019. Truss morfometrik dan karakter meristik ikan kelabau (Osteochilus mela-nopleurus Bleeker, 1852) dari tiga popu-lasi di Sungai Kampar, Sungai Siak, dan Sungai Rokan, Provinsi Riau. Jurnal Iktiologi Indonesia, 19(2): 283-295.
Cahyono RN, Budiharjo A, Sugiyanto. 2018. Keragaman dan pengelompokan ikan berdasarkan karakter morfologi di eko-sistem Bendungan Colo Sukoharjo Jawa Tengah. Depik, 7(1): 9-21.
Carpenter KE, Niem VH (eds). 1999. FAO species identification guide for fishery purpose. The living marine resources of the Western Central Pacific. Volume 3. FAO, Roma. pp. 1397-2068.
Carrier JC, Musick JA, Heithaus MR. 2010. Shark and Their Relatives II: Biodiver-sity, Adaptive Physiology and Conser-vation. CRC Press Boca Raton London New York. 746 p.
Faizah U. 2008. Karakteristik marka genetik DNA mitokondria sebagai acuan kon-servasi genetik harimau Sumatera. Tesis. Sekolah Pascasarjana. Institut Pertanian Bogor.
Freeland JR. 2005. Molecular ecology. John Wiley & Sons Ltd. Chichester, England. 402 p.
Genisa AS. 1999. Pengenalan jenis-jenis ikan laut ekonomi penting di Indonesia. Oseana, 24(1): 17-38.
Graham CA, Hill AJM. 2001. DNA sequencing protocols. Second edition. Humana Press. Totowa, New Jersey. 239 p.
Hebert PDN, Ratnasingham S, de Waard JR. 2003. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. In: Barret SFRS (ed.). Proceedings of the Royal Society B: Biological Science, 270 (Suppl 1): S96-S99.
Klug WS, Cummings MR. 1994. Concept of genetics. 4th ed. Prentice Hall, Engle-wood. 779 p.
Kusuma AB, Bengen DG, Madduppa HH. Subhan B, Arafat D. 2016. Keanekara-gaman genetik karang lunak Sarcophyton trocheliophorum pada populasi Laut Ja-wa, Nusa Tenggara dan Sulawesi. Jurnal Enggano, 1(1): 89-96.
Mackie IM, Pryde SE, Gozales-Sotelo C, Me-dina I, Perez-Martin R, Quinteiro J, Rey Mendez M, Rehbein H. 1999. Challenges in the identification of species of canned for fish. Trends in Food Science and Technology, 10 (1): 9-14.
Nei M. 1972. Genetic distance between popula-tion. American Naturalist, 106(949): 283-292.
Nei M. 1987. Moleculer evolutionary genetics. Columbia University Press, New York. 512 p.
Heemstra P, Heemstra E. 2004. Coastal fishes of Southern Africa. National Inquiry and Service Centre. Grahamstown, South Africa. 488 p.
Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhi-bitors. Proceeding of the National Aca-demy of Sciences of the United States of America, 74(12): 5463-5467.
Sambrook J, Russel DW. 2001. Molecular Clo-ning a Laboratory Manual 1st edition. Publisher Cold Spring Harbor Laboratory Press. 999 p.
Sneath PH, Sokal RR. 1973. Numerical taxono-my. The principles and practice of nume-rical classification. 1st edition. WH Free-man and Company, San Francisco. 573 p.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: mole-cular evolutionary genetics analysis using maximum likehood, evolutionary distance, and maximum parsimony method. Molecular Biology and Evolu-tion, 28(10): 2731-2739.
Tallei, TE, Rembet RE, Palealu JJ, Kolondam BJ. 2016. Sequence variation and phylo-genetic analysis of Sansevieria trifas-ciata (Asparagaceae). Bioscience Re-search, 13(1): 1-7.
Varghese AS. 2005. Systematics and biology of fishes of the family Hemiramphidae of Cochin Coast. Thesis. School of Indus-trial Fisheries. Cochin University of Science and Technology. Cochin. 360 p.
Wuaten JF, Reppie E, Labar LI. 2011. Kajian perikanan tangkap ikan julung-julung (Hyporhamphus affinis) di Perairan Kabupaten Kepulauan Sangihe. Jurnal Perikanan dan Kelautan Tropis, 7(2): 80-86.
Wong LL. 2011. DNA barcoding and related moleculer markers for fish species authentication. Phylogenetic Assessment and Population Studies. Auburn Univer-sity. Auburn. Alabama. 118 p.