OPEN ACCESS
PEER-REVIEWED |
RESEARCH ARTICLE
EFFECT OF DRUG-METABOLIZING ENZYME ACTIVITY INDUCED BY POLYCHLORINATED BIPHENYL ON THE DURATION OF OXYTETRACYCLINE IN CARP
Article Sidebar
How to Cite
The article can trace at:
Main Article Content
Abstract
A study was made of the induction of drug-metabolizing enzyme activity and the effect of the induced activity on the duration of oxytetracycline (OTC) in carp, Cyprinus carpio. Carp were fed a diet containing polychlorinated biphenyl (PCB)(0,1 mg/100 g body weight/day) for 2 week to induce drug-metabolizing enzymes. After pretreatment, OTC was administered in feed to PCB-treated and control carp as a single dose of 50mg/kg body weight.The result demonstrate that PCB highly induses benzo (a) pyrene hydroxylase among the enzymes of carp, but the induced hydroxylase does not react to OTC, resulting in no change in their tissue levels and duration time.
Downloads article
Article Details
Copyright (c) 2017 Jurnal Iktiologi Indonesia |
|
|
References
Cantfort, V.J.; Graeve, J.D. & Gielen, J.E. 1977. Radioactive assay for aryl hydrocarbon hydroxylase. Improved method and biological importance. Biochem. Biophys Res. Com., 79: 505-512.
Bend, J.R.; James, M.O. & Dansette, D.M. 1976. In vitro metabolism of xenobiotics in some marine animals. Ann. N.Y. Acad. Sci., 298: 505.
Bend, J.R. & James, M.O. 1979. Xenobiotic metabolism in marine and freshwater species. Biochem. Biophys. Perspect. Mar. Biol, 4:125-188.
Brodie, B.B. & Maickel, R.P. 1962. Comparative biochemistry of drug metabolism. Proc. First. Int. Pharmacol. Meet., 6: 299-324.
Chambers, J.E. & Yarbrough, J.D. 1979. A seasonal study of microsomal mixed-function oxidase components in insecticide resistant and susceptible mosquito fish, Gambusia affinis. Toxicol. Appl. Pharmacol., 48: 497-507.
Conney, A.H. 1977. Pharmacological implications of microsomal enzyme induction. Pharmacol. Rev., 19:317-364.
Guengerich, F.P. 1988. Cytochrome P-450. Comp. Biochem. Physiol., 89C (1): 1-4.
Habig, W.H.; Pabst, M.J. & Jakoby, W.B. 1974. Glutathione s-transferases. The fist enzymatic step in mercapturic acid formation. J. Biol. Chem., 249 (22): 71307139.
Hodgson, E. & Kulkarni, A.P. 1984. Mechanism of pesticide action. (G.K. Kohn, Ed). Amer. Chem. Soc., Symposium Ser. 2.
IIIing, H.P.A. & Benford, D. 1976. Observation on the accessibility of acceptor substrates to the active centre of UDP-glucuronosyl-transferase in vitro. Biochim. Biophys. Acta, 429: 768-779.
Kobayashi, K.; Oshima, Y.; Hamada, S. & Taguchi, C. 1987. Induction of phenol-sulfate conjugation activity by exposure to phenols and duration of its induced activity in shortnecked clam. Bull. Jap. Soc. Sci. Fish, 53 (11): 2073.
Lowry, O.H.; Rosebrough, N.J.; Farr, A.L. & Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem, 193: 265-275.
Lumban Batu, D.F. 1991. A study on the characteristics of drug-metabolizing enzyme and its relation to the changes in duration of drugs in fishes. Doctor Degree Dissertation. Kyushu University. Fukuoka, Japan.
Lumban Batu, D.F. 1992. Induction of the cytochrome P-450 dependent mixed function oxidases in carp by dietary administration of environmental contaminants. Paper presented to : The Regional Seminar on Waste treatment and recovery technology and the sixth NCPR meeting wastenet. Bogor, Indonesia. Agust 25-26, 1992.
Lumban Batu, D.F. 1995. Induction capabilities of some environmental contaminants on the enzyme activities of carp microsomes and cytosol fraction. Scientific Journal Gakuryoku, 2: 31-43.
Lumban Batu, D.F. 1996. Struktur, evolusi, dan pengaturan gen-gen P-450. Diktat Kuliah Ekotoksikologi Laut Lanjutan II. Program Studi Ilmu Kelautan. Program Pasca Saijana. Institut Pertanian Bogor. 73 pp.
Lumban Batu, D.F. 2001a. Effect of several environmental contaminants on the benzo(a)pyrenehydroxylase enzyme activity of carp microsome. Indonesian Journal of Aquatic Sciences and Fisheries, VIII (1): 23-29.
Lumban Batu, D.F. 2001b. Induction of several environmental contaminants on the cytochrome P-450 content of carp microsome and cytosol fractions. Indonesian Journal of Aquatic Sciences and Fisheries, VIII (2): 9-18.
Lumban Batu, D.F. 2002a. Determination of residual thiamphenicol in fishes by High Performance Liquid Chromatography. Indonesian Journal of Aquatic Science and Fisheries, IX (1): 35-42.
Lumban Batu, D.F. 2002b. Comparison of tissue level of oxolinic acid in fresh and sea water fishes after oral administration. Indonesian Journal of Aquatic Sciences and Fisheries, IX (1): 53-58.
Lumban Batu, D.F. 2002c. Determination of residual of oxytetracycline in fishes by high perfomance liquid after ehromatography oral administration. Indonesian Journal of Aquatic Sciences and Fisheries, IX (1):
Nebert, D.W.; Asdenik, M.; Conn, M.J.; Estabrook, R.W. Gonzales, F.J.; Guengerich, F.P.; Gunsalus, I.C.; Johnson, E.F.; Kemper, B.; Levin, W.; Philips, I.R.; Sato, R. & Watterman, M.R.. 1987. The P-450 gene superfamily. Recommended Nomenclature DNA, 6: 1-11.
Omura, T. & Sato, R. 1964. Carbon monooxide-binding pigment of liver microsomes. J. Biol. Chem., 239 (7): 2370-2378.
Ueno, R.; Uno, K.; Kubota, S.S. & Horiguchi, Y. 1989. Determination of oxytetracycline in fish tissues by high performance liquid chromatography. Nippon Suisan Gakkaishi, 55 (7): 1273-1276.