OPEN ACCESS PEER-REVIEWED | RESEARCH ARTICLE

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Authors

Siti Aslamyah
Zainuddin Zainuddin
Badraeni Badraeni

Abstract

The use of combinations of microorganisms as feed additives can synergize to produce enzymes to help the digestive process of feed. This study aims to determine the best combination of microorganisms as probiotics in feeding on growth performance, gastric evacuation, and blood glucose levels of milkfish. Milkfish with an initial weight of 9.21±0.14 g, reared at a density of 20 fish in an aquarium measuring 50 x 40 x 35 cm3 containing 48 L of water with 20 ppt salinity and equipped with a recirculation system. The research design was Completely Randomized Design with five treatments, namely: control (feed without microorganisms), Bacillus sp. and Lactobacillus sp., Rhizophus sp. and Aspergillus sp., Saccharomyces sp. and Trichoderma sp., and Mixed microorganisms, Bacillus sp., Lactobacillus sp., Rhizophus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. During 50 days of rearing, the fish were given experimental feed three times a day at 07.00, 12.00, and 15.00 at 5% body weight. The results showed that the treatment significantly affected growth, feed efficiency, and hepatosomatic index. However, it did not affect milkfish's survival and liver and muscle glycogen levels. Absolute growth (30.79±0.87 g), relative growth rate (333.91±5.83%), biomass growth (615.76±17.48%), feed efficiency (57.26±0.39 %), and the best hepatosomatic index (0.99±0.01) were obtained in the mixed microorganism treatment, and mg/g, and muscle 5.45-5.72 mg/g. The gastric evacuation rate and the fastest peak and peak decrease in blood glucose levels were obtained in the mixed microorganisms treatment at 5 and 3-4 hours postprandial. The diverse microorganisms can be added to the feed at a dose of 10 mL/kg to support the intensification of milkfish culture


Abstrak


Pemanfaatan kombinasi mikroorganisme sebagai aditif pakan dapat saling bersinergi menghasilkan enzim untuk membantu proses pencernaan pakan. Penelitian ini bertujuan menentukan kombinasi mikroorganisme terbaik sebagai probiotik dalam pakan terhadap kinerja pertumbuhan, laju pengosongan lambung, dan kadar glukosa darah ikan bandeng. Ikan bandeng dengan bobot awal 9,21±0,14 g dipelihara dengan kepadatan 20 ekor pada akuarium berukuran 50 x 40 x 35 cm3 yang berisi 48 L air bersalinitas 20 ppt dan dilengkapi sistem resirkulasi. Penelitian didesain dalam rancangan acak lengkap dengan lima perlakuan, yaitu: kontrol (pakan tanpa mikroorganisme), Bacillus sp. dan Lactobacillus sp., Rhizophus sp. dan Aspergillus sp., Saccharomyces sp. dan Trichoderma sp., serta kombinasi mikroorganisme Bacillus sp., Lactobacillus sp., Rhizophus sp., Aspergillus sp., Saccharomyces sp., dan Trichoderma sp. Selama 50 hari pemeliharaan, ikan diberi pakan percobaan 3 kali sehari yakni pukul 07.00, 12.00, dan 15.00 sebanyak 5% dari bobot badan. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata terhadap pertumbuhan, efisiensi pakan, dan indeks hepatosomatik. Namun, tidak berpengaruh terhadap sintasan, kadar glikogen hati dan otot ikan bandeng. Pertumbuhan mutlak (30,79±0,87 g), laju pertumbuhan relatif (333,91±5,83%), pertumbuhan biomassa (615,76±17,48%), efisiensi pakan (57,26±0,39%), dan indeks hepatosomatik (0,99±0,01) terbaik diperoleh pada perlakuan kombinasi mikroorganisme dan terendah pada kontrol. Kisaran sintasan yang dihasilkan 93,33-100%, glikogen hati 6,60-6,89 mg/g, dan otot 5,45-5,72 mg/g. Laju pengosongan lambung, serta mencapai puncak dan penurunan puncak kadar glukosa darah tercepat pada perlakuan kombinasi mikroorganisme pada jam ke 5 dan jam ke 3-4 post prandial. Untuk mendukung intensifikasi budidaya ikan bandeng, kombinasi mikroorganisme dapat ditambahkan dalam pakan dengan dosis 10 mL/kg pakan.

Keywords:
Microorganisms; probiotics; feed, growth; milkfish;

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References

Arief M. 2013. Pemberian probiotik yang berbeda pada pakan komersil terhadap pertumbuhan retensi protein dan serat kasar pada ikan nila (Oreochromis sp.). Argoveteriner, 1(2): 88-93.


Afrizal A, Purwanto A. 2011. Pemanfaatan selulosa bakterial nata de coco sebagai adsorban logam Cu (Ii) dalam sistem berpelarut air. Jurnal Riset Sains dan Kimia Terapan, 1(1): 27-32.


Aslamyah S. 2006. Peningkatan Peran Mikroba Saluran Pencernaan untuk Memacu Pertumbuhan Ikan Bandeng. Disertasi. Program Pascasarjana, Institut Pertanian Bogor. Bogor.


Aslamyah S. 2011. Effect of microbe Bacillus sp. and Carnobacterium sp. as feed additive on glukose content in blood, metabolic rate and energy balance in the omnivores phase giant gouramy, Osphronemus gourami Lac. Prosiding Seminar Nasional Perikanan dan Kelautan Pekanbaru, Riau, 26-27 Oktober 2011. ISBN 978-979-792-286-3.


Aslamyah S, Karim MY, Mirna. 2015. Konsumsi oksigen dan metabolisme ikan bandeng (Chanos chanos Forsskal) pada berbagai konsentrasi Lactobacillus sp. Prosiding Seminar Nasional Tahunan X Hasil Penelitian Perikanan dan Kelautan UGM, 8 Agustus 2015. ISBN: 978-602-71759-1-4.


Aslamyah S, Karim MY, Badraeni. 2017. Fermentasi tepung rumput laut dengan berbagai fermentor untuk meningkatkan kualitas sebagai bahan baku pakan ikan. Jurnal Akuakultur Indonesia, 16(1):11-17.


Aslamyah S, Karim MY, Badraeni, Tahya AM. 2018. Effect of Fermented Seaweed Addition on Blood Glucose Level, Hepatosomatic Index, and Gastric Evacuation Rate of Milkfish Chanos chanos Forsskal Larvae. AACL Bioflux, 11(1) 226-231.


Chilmawati D, Swastawati F, Wijayanti I, Ambaryanto, Cahyono B. 2018. Probiotik guna peningkatan pertumbuhan, efisiensi pakan, tingkat kelulushidupan dan nilai nutrisi ikan bandeng (Chanos chanos). Indonesian Journal of Fisheries Science and Technology (IJFST), 13(2): 119-125.


Cruz PM, Ibanez AL, Hermosillo OAM, Saad HCR. 2012. Use of Probiotic in Aquaculture. ISRN Microbiology.


Dommels YEM, Kemperman RA, Zebregs YEMP, Draaisma RB. 2009. Survival of Lactobacillus reuteri dsm 17938 and Lactobacilus rhamnosus gg in the human gastrointestinal tract with daily consumption of a low-fat probiotic spread. Applied and Environmental Microbiology, 75(19): 6198-204.


Encarnação, P. 2016. Functional feed additives in aquaculture feeds. In Aquafeed formulation. Academic Press p: 217-237.


Ikram-ul-Haq MMJ, Khan S, Siddiq Z. 2005. Cotton saccharifying activity of cellulases produced by co-culture of Aspergillus niger and Trichoderma viride. Research Journal of Agriculture and Biological Sciences, 1(3): 241-245.


Khasani I. 2007. Aplikasi probiotik menuju sistem budidaya perikanan berke-lanjutan. Media Akuakultur, 2(2): 1-3


Kindom T, Alisson ME. 2010. The fecundity, gonadosomatic and hepatosomatic indices of Pellonula leonensis in the Lower Nun River, Niger Delta, Nigeria. Current Research Journal of Biological Science, 3(2): 175-179.


Lee SM, Hwang UG, Cho SH. 2000. Effects of feeding frequency and dietary moisture content on growth, body composition and gastric evacuation of juvenile Korean rockfish (Sebastes schlegeli). Aquaculture, 187: 399-409.


Matthews JO, Higbie AD, Southern LL, Coombs DF, Bidner TD, Odgaard RL. 2003. Effect of chromium propionate and metabolizable energy on growth, carcass traits, and pork quality of growing-finishing pigs. Journal of Animal Science. 81: 191-196.


NRC. 1988. Designing Food, Animal Product Option in the Market Place. National Research Council, Academy Press, Washington. DC.


Putri DR, Agustono, Subekti S. 2012. Kandungan bahan kering, serat kasar dan protein kasar pada daun lamtoro (Leucaena glauca) yang difermentasi dengan probiotik sebagai bahan pakan alami. Jurnal Ilmiah dan Kelautan, 4(2), 161-167.


Ramadhana S, Fauzana NA, Ansyari P. 2012. Pemberian pakan komersil dengan penambahan probiotik yang mengandung Lactobacillus sp. terhadap kecernaan dan pertumbuhan ikan nila (Oreochromis niloticus). Fish Scientiae, 2(42):178-187.


Sakamole E, Lumenta TC, Runtuwene M. 2014. Pengaruh pemberian probiotik dosis berbeda dalam pakan terhadap pertumbuhan dan konversi pakan benih ikan mas (Cyprinus carpio). Buletin Sariputra, 1(1): 29-33.


Shitandi A, Alfred M, Symon M. 2007. Probiotic characteristic of lactococcus strainfrom local fermented Amaranthus hybrydus and Solanum nigrum. African Crop Science Conference Proceedings, 8:1809-1812.


Steinkraus KH. 1983. Handbook of Fermented Foods. Marcel Dekker. New York


Syamsuddin R. 2010. Sektor Perikanan Kawasan Indonesia Timur: Potensi Permasalahan, dan Prospek. PT Perca, Jakarta.


Takeuchi T. 1988. Laboratory Work Chemical Evaluation of Dietary Nutrients. In: Watanabe, T. (ed.): Fish Nutrition and Mariculture. Department of Aquatic Biosience. Tokyo University of Fisheries. JICA p:179-226


Wahyuningtyas P, Sitasiwi AJ, Mardiati SM. 2018. Hepatosomatic index (HIS) dan diameter hepatosit mencit (Mus musculus L.) setelah paparan ekstrak air biji pepaya (Carica papaya L.). Jurnal Biologi, 7(1): 8-17.


Wang MQ, Xu ZR, Sun JY, Kim BG. 2008. Effects of enzyme supplementation on growth, intestinal content viscosity, and digestive enzyme activities in growing pigs fed rough rice-based diet. Asian-Aust. Journal of Animal Science, 21(2): 270-276.


Wedemeyer GA, Yasutake WT. 1977. Clinical methods for the assesment of the effects of environmental stress on fish health. Technical Paper of the US Fish and Wildlife Service. Volume 89. USA Washington DC: US Department of the Interior Fish and Wildlife Service.


Yandes Z, Affandi, R. (2003). Pengaruh pemberian selulosa dalam pakan terhadap kondisi biologis benih ikan gurami (Osphronemus gourami Lac). Jurnal Iktiologi Indonesia, 3(1): 27-33.


Zhang YHP, Himmel ME, Mielenz JR. 2006. Outlook for cellulase improvement: screening and selection strategies. Biotechnology Advances, 24(5): 452-481.


Zonneveld N, Huisman EA, Boon JH. 1991. Prinsip-Prinsip Budidaya Ikan. Gramedia, Jakarta.