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Induction on gonadal maturation of male striped catfish Pangasianodon hypopthalmus (Sauvage, 1878) using Javanese long pepper extract Piper retrofractum Vahl. enriched feed
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Abstract
Javanese long pepper is an aphrodisiac plants that have hormonal effects. This study aimed to evaluate the using of Javanese long pepper extract (JLPE) enriched feed to accelerate the gonadal maturation of male striped catfish. The dose 37.5 and 187.5 mg kg body weight-1 day-1 JLPE enriched feed were given on treatments compared to 17-a methyl testosterone (50 ^g kg body weight-1 week-1) and control. The treatments were given for 8 weeks on male striped catfish fish weighed 250±18.6 g. Gonadosomatic index of JLPE treatment higher than control since the second week (p<0.05), also testosterone levels in 187.5 mg kg body weight-1 day-1 JLPE treatment to control (p<0.05). The spermatozoa dispersion reached 75% in JPLE treatment higher than control (p<0.05), althought there was not significant difference on spermatocrite level (p> 0.05). Therefore, JLPE treatment at dose 187.5 mg kg body weight-1 day-1 increased the reproductive performance and sperm quality of male striped catfish.
Abstrak
Cabe jawa merupakan salah satu tanaman yang memiliki efek hormonal sebagai afrodisiak. Penelitian ini bertujuan un-tuk mengevaluasi pemberian ekstrak cabe jawa (ECJ) melalui pakan terhadap akselerasi pematangan gonad ikan patin siam jantan. Perlakuan yang diberikan meliputi ECJ dengan dosis 37,5 dan 187,5 mg kg ikan-1 hari-1, dibandingkan dengan 17-a Metiltestosteron (50 ^g kg ikan-1 minggu-1) dan kontrol selama 8 minggu. Perlakuan ECJ menunjukkan in-deks kematangan gonad lebih tinggi dibandingkan kontrol sejak minggu ke-2 (p<0,05). Kadar testosteron darah pada perlakuan ECJ 187,5 mg kg ikan-1 hari-1 lebih tinggi dibandingkan kontrol (p<0,05). Pada minggu ke-8, sebaran spermatozoa perlakuan ECJ mencapai 75%, sedangkan sebaran spermatozoa kontrol kurang dari 50%. Kepadatan, volume, dan motilitas sperma perlakuan ECJ dan 17a-metiltestosteron lebih tinggi dibandingkan dengan kontrol (p<0,05) pada minggu ke-8, namun kadar spermatokrit menunjukkan hasil yang sama (p>0,05). Perlakuan ECJ 187,5 mg kg ikan'1 hari-1 meningkatkan performa reproduksi dan kualitas sperma ikan patin siam jantan.
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