DNA barcoding application of garfish (Hemirhampus sp.) in North Maluku Sea


M. Janib Achmad, Martini Djamhur, M. Abjan Fabanyo, Nebuchadnezzar Akbar
 DOI  https://doi.org/10.32491/jii.v19i3.506

Abstract

The purpose of this study was to identify the species of garfish in the waters of north Maluku by applying DNA bar-coding techniques. The method used in the study was the primary design of DNA extraction and isolation, and DNA amplification through polymerase chain reaction. The results showed that Gen Cyt b analysis amplified ± 350 bp amplicon and for the alignment analysis showed that the amplification sample sequence had similarity with the Cyt b gene sequence of Hemiramphus balao with value of 97%. Sequence analysis showed that the six specimens had very low genetic distances. It can be concluded that the isolate samples of garfish have a very close kinship.

Keywords

barcoding; cytochrome; garfish; molecular

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