Evaluation of monosodium glutamate suplementation on physiological response, growth performance, and feed utilization in North African catfish Clarias gariepinus (Burchell, 1822)

Agustinus Ngaddi, Dedi Jusadi, Wasjan Wasjan, Eddy Supriyono
 DOI  https://doi.org/10.32491/jii.v19i3.500


A triplicate experiment was conducted to evaluate the supplementation of monosodium glutamate into the diet on physiological responses, growth performances, and feed efficiency of north African catfish Clarias gariepinus cul-tured in high ammonia environment. A hundred fish with an initial body weight of 11.9±0.3 g was rearing for 60 days without any water exchange in nine plastic tanks (1x1x1 m3) at experimental pond of Department of Aquaculture, Bogor Agricultural University. During rearing period, fish were fed on the diet supplemented with 0%, 0.87%, or 1.74 % of monosodium glutamate, two times a day at satiation. Result shows that the supplementation of monoso-dium glutamate in feed stimulates change in fish physiological responses such as lower Alanin Transaminase mono-sodium glutamate enzyme activity, lower blood ammonia, and higher intestinal glutamine. Feeding using monoso-dium glutamate-supplemented feed at three different doses results in the same growth rate. However, the highest feed efficiency of North African catfish was recorded in the treatment of feed supplemented with 0.87% monosodium glutamate. Thus, it can be inferred that the usage of monosodium glutamate may improve physiological response and feed efficiency but does not affect fish growth rate.


Clarias gariepinus; feed efficiency; growth; monosodium glutamate; physiological response

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Al-Mamary M, Al-Habori M, Al-Aghbari AM, Baker MM. 2002. Investigation into the toxicological effects of Catha edulis leaves: a short term study in animals. Phytotheraphy Research, 16(2): 127-132.

[APHA] American Public Health Association. 1998. Standard methods for the exami-nation of water and waste water. Ame-rican Public Health Association (APHA). American Water Works Association (AWWA) and Water Pollution Control Federation (WPCF). 20th ed. Washing-ton. 4: 114 p.

Caballero-Solares A, Viegas I, Salgado MC, Siles AM, Sáez A, Metón I, Baanante IV, Fernández F. 2015. Diets supplemented with glutamate or glutamine improve protein retention and modulate gene expression of key enzymes of hepatic metabolism in gilthead seabream (Sparus aurata) juveniles. Aquaculture, 444: 79-87.

Chen JC, Kou YZ. 1993. Accumulation of ammonia in the haemolymph of Penaeus monodon exposed to ambient ammonia. Aquaculture, 109(2): 177-185.

Chew SF, Ip YK. 2014. Excretory nitrogen metabolism and defence against am-monia toxicity in air-breathing fishes. Journal of Fish Biology, 84(3): 603-638.

Choudhury MG, Saha N. 2012. Influence of en-vironmental ammonia on the production of nitric oxide and expression of induci-ble nitric oxide synthase in the fresh-water air-breathing catfish (Heteropneus-tes fossilis). Aquatic Toxicology, 116-117: 43-53.

Cooper AJ, Plum F. 1987. Biochemistry and physiology of brain ammonia. Physiolo-gical Reviews, 67(2): 440-519.

de la Torre FR, Salibián A, Ferrari L. 2000. Bio-markers assessment in juvenile Cyprinus carpio exposed to waterborne cadmium. Environmental Pollution, 109(2): 277-282.

Diniz YS, Fernandes AAH, Campos KE, Mani F, Ribas BO, Novelli ELB. 2004. Toxicity of hypercaloric diet and mono-sodium glutamate: oxidative stress and metabolic shifting in hepatic tissue. Food and Chemical Toxicology, 42(2): 313-319.

Farombi EO, Onyema OO. 2006. Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: modulatory role of vitamin C, vitamin E and quercetin. Human and Experimental Toxicology, 25(5): 251-259.

Fauji H, Budiardi T, Ekasari J. 2018. Growth performance and robustness of African catfish Clarias gariepinus (Burchell) in biofloc-based nursery production with different stocking densities. Aquaculture Research, 49(3): 1339-1346.

Hegazi MM, Hasanein SS. 2010. Effects of chronic exposure to ammonia concentra-tions on brain monoamines and ATPases of Nile tilapia (Oreochromis niloticus). Comparative Biochemistry and Physiolo-gy Part C, 151(4): 420-425.

Jürss K, Bastrop R. 1995. Amino acid metabolism in fish. In: Hochachka PW, Mommsen TP (eds.). Biochemistry and Molecular Biology of Fishes, Volume 4. Elsevier, Amsterdam. pp. 159-189.

Lemaire P, Drai P, Mathieu A, Lemaire S, Car-rière S, Giudicelli J, Lafaurie M. 1991. Changes with different diets in plasma enzymes (GOT, GPT, LDH, ALP) and plasma lipids (cholesterol, triglycerides) of sea-bass (Dicentrarchus labrax). Aquaculture, 93(1): 63-75.

Li C, Zhang M, Li M, Zhang Q, Qian Y, Wang R. 2018. Effect of dietary alanyl-gluta-mine dipeptide against chronic ammonia stress induced hyperammonemia in the juvenile yellow catfish (Pelteobagrus fulvidraco). Comparative Biochemistry and Physiology Part C, 213: 55-61.

Lin Y, Zhou XQ. 2006. Dietary glutamine sup-plementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian). Aquaculture, 256(1-4): 389-394.

Miron DS, Moraes B, Becker AG, Crestani M, Spanevello R, Loro VL, Baldisserotto B. 2008. Ammonia and pH effects on some metabolic parameters and gill histology of silver catfish, Rhamdia quelen (Hepta-pteridae). Aquaculture, 277(3-4): 192-196.

Newsholme P, Procopio J, Lima MMR, Pithon-Curi TC, Curi R. 2003. Glutamine and glutamatetheir central role in cell meta-bolism and function. Cell Biochemistry and Function, 21(1): 1-9.

[NRC] National Research Council. 2011. Nu-trient requirements of fish and shrimp. The National Academies Press, Washing-ton, D.C. 376 p.

Randall DJ, Tsui TKN. 2002. Ammonia toxicity in fish. Marine Pollution Bulletin, 45(1-12): 17-23.

Rose C. 2006. Effect of ammonia on astrocytic glutamate uptake/release mechanisms. Journal of Neurochemistry, 97(s1): 11-15.

Saha N, Datta S, Bhattacharjee A. 2002. Role of amino acid metabolism in an air-breath-ing catfish, Clarias batrachus in respon-se to exposure to a high concentration of exogenous ammonia. Comparative Bio-chemistry and Physiology Part B, 133(2): 235-250.

Saha N, Datta S, Kharbuli ZY, Biswas K, Bhat-tacharjee A. 2007. Air-breathing catfish, Clarias batrachus upregulates glutamine synthetase and carbamyl phosphate syn-thetase III during exposure to high ex-ternal ammonia. Comparative Bioche-mistry and Physiology Part B, 147(3): 520-530.

Sinha AK, Giblen T, AbdElgawad H, De Rop M, Asard H, Blust R, De Boeck G. 2013. Regulation in amino acid metabolism as a defensive strategy in the brain of three freshwater teleosts in response to high environmental ammonia exposure. Aqua-tic Toxicology, 130-131: 86-96.

Sogbesan OA, Ahmed YM, Ajijola KO. 2017. Growth performance, nutrient utilization, somatic indices and cost benefit analyses of african basil leaf additive diets on Clarias gariepinus (Burchell, 1822) fingerlings. Journal of Animal Research and Nutrition, 2(1): 10.

Steel RDG, Torrie JH. 1993. Principles and procedures of statistics. A biometrical approach. McGraw-Hill Book Company Inc., New York, NY, 633 p.

Tapiero H, Mathé G, Couvreur P, Tew KD. 2002. II. Glutamine and glutamate. Bio-medicine and Pharmacotherapy, 56(9): 446-457.

Vangen B, Hemre GI. 2003. Dietary carbohy-drate, iron and zinc interaction in Atlan-tic salmon (Salmo salar). Aquaculture, 219(1-4): 597-611.

Wang Y, Walsh PJ. 2000. High ammonia tole-rance in fishes of the family Batrachoid-idae (Toadfish and Midshipmen). Aqua-tic Toxicology, 50(3): 205-219.

Wilkie MP. 2002. Ammonia excretion and urea handling by fish gills: present under-standing and future research challenges. Journal of Experimental Zoology, 293 (3): 284-301.

Wright PA, Steele SL, Huitema A, Bernier NJ. 2007. Induction of four glutamine syn-thetase genes in brain of rainbow trout in response to elevated environmental am-monia. Journal of Experimental Biology, 210(16): 2905-2911.

Zhao Y, Hu Y, Zhou XQ, Zeng XY, Feng L, Liu Y, Jiang WD, Li SH, Li DB, Wu XQ, Wu CM, Jiang J. 2015. Effects of dietery glutamate supplementation on growth performance, digestive enzyme activities and antioxidant capacity in intestine of grass carp (Ctenopharyngodon idella). Aquaculture Nutrition, 21(6): 935-941.

Zonneveld N, Huisman EA, Boon JH. 1976. Prinsip-prinsip budidaya ikan. Gramedia Pustaka Utama, Jakarta.

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