OPEN ACCESS PEER-REVIEWED | RESEARCH ARTICLE

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Authors

M. Agus Suprayudi
Wastu Dimahesa
Dedi Jusadi
Mia Setiawati
Juli Ekasari

Abstract

This research was conducted to evaluate the effect of supplementation of crude enzyme from rumen fluid in the natural protein-based diet for the growth performance of nile tilapia. The weight average of nile tilapia juvenile which used in this research was 6.10±0.49 g. The fish were reared in the aquarium with measuring 35 x 40 x 50 cm3 with a density of eight fish per aquarium for 54 days. Aquariums equipped with recirculation system. Fish were fed ad libitum three times a day. Four types of feed that used in this research have the similar protein content (28% and 4,050 kcal GE kg-1). All feed were supplemented with different level of crude enzyme namely 0 ml kg-1 (diet A), 200 ml kg-1 (diet B), 400 ml kg-1 (diet C) and 600 ml kg-1 (diet D). Food consumption, feed efficiency, protein retention rate, lipid retention, daily growth, and survival rate were used as parameters tested. Research design was complete randomized block design with four treatments and each of the treatment with three replications. Research results showed that supplementation of crude enzyme from rumen fluid have significant effect on feed efficiency, protein retention and lipid retention of nile tilapia (p<0.01). The effect of crude enzyme supplementation was not significant on survival rate, specific growth and feed consumption, but supplementation of 200 ml kg-1 crude enzyme showed the best effect on the growth performance of nile tilapia. AbstrakPenelitian ini dilakukan untuk mengevaluasi pengaruh suplementasi crude enzim cairan rumen pada pakan berbasis protein nabati terhadap kinerja pertumbuhan ikan nila (Oreochromis niloticus). Ikan yang digunakan dalam penelitian ini adalah juwana ikan nila yang berukuran 6,10±0,49 g. Ikan dipelihara dalam akuarium berukuran 35x40x50 cm3 de-ngan kepadatan delapan ekor per akuarium, yang dilengkapi dengan sistem resirkulasi selama 54 hari. Ikan diberi pakan sampai kenyang dengan frekuensi tiga kali sehari. Empat macam pakan yang digunakan dalam penelitian ini memiliki kadar protein dan energi yang sama yakni 28% dan 4.050 kkal GE kg-1 dan berbeda pada jumlah penambahan crude enzim cairan rumen domba (crude enzyme) yakni 0 ml kg-1 (pakan A), 200 ml kg-1 (pakan B), 400 ml kg-1 (pakan C) dan 600 ml kg-1 (pakan D). Konsumsi pakan, efisiensi pakan, laju retensi protein, retensi lemak, pertumbuhan harian, dan kelangsungan hidup digunakan sebagai paramer yang diuji. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap dengan empat perlakuan dan setiap perlakuan diulang tiga kali. Hasil penelitian ini memper-lihatkan bahwa penambahan crude enzim cairan domba memberi pengaruh yang nyata terhadap efisiensi pakan, retensi protein dan retensi lemak ikan nila (p<0,01). Adapun nilai kelangsungan hidup, laju pertumbuhan spesifik dan konsumsi pakan terlihat tidak berbeda akibat adanya penambahan crude enzim tersebut. Penambahan enzim sebanyak 200 ml kg-1 memberikan hasil yang terbaik terhadap kinerja pertumbuhan ikan nila.

Keywords:
crude enzyme , Oreochromis niloticus , growth , plant protein.

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