Main Article Content


Alam Putra Persada
Dedy Duryadi Solihin
Ridwan Affandi


DNA barcoding based on partial Cytochrome Oxidase subunit I (COI) gene in the mitochondrial has widely used in species identification and biodiversity studies. COI gene application is expected to obtain genetic characteristic, genetic variations and phylogeny of giant featherback. The aim of this research was to analyze genetic diversity of giant featherback Chitala lopis in Lampung and Kalimantan. To analyse genetic distance, Kimura two parameter (K2P) model was performed where to determine nucleotide variation & polymorphism and also reconstructed of phylogenetic tree was used MEGA 7.0 software. Total nine individuals were obtained from three populations, i.e. Lampung, West Kalimantan and South Kalimantan. The results showed that giant featherback has 689 bp conserve, 18 bp variation, 13 bp parsimony-informative, and 2 bp singleton sites from 707 bp COI partial gene. The average within-species, in-group, and out-group based on K2P distances were 1.24%, 1.43% & 1.58% (AP008922.1; KM213054.1), and 13.00% respectively. The Single Nucleotide Polymorphism (SNP) was obtaining from 13 SNP sites. West Kalimantan samples have two SNP (471 and 528 site). The South Kalimantan samples showed more specific nucleotides with nine SNP (120, 129, 144, 201, 306, 324, 474, 615 and 644). Based on genetic distance, the biggest difference was in the South Kalimantan sample (1.58%) compared with Lampung and West Kalimantan. The results of the K2P neighbour-joining phylogenetic tree reconstruction show that the South Kalimantan samples are in a different group. The West Kalimantan sample shows that it is closely related to the Lampung.

COI , DNA barcoding , giant featherback , SNP

Downloads article

Download data is not yet available.

Article Details


Copyright (c) 2021 Jurnal Iktiologi Indonesia

  Authors who publish with this journal agree to the following terms:  
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  
  b.) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.  
  c.) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).  
  Creative Commons License
Jurnal Iktiologi Indonesia by Masyarakat Iktiologi Indonesia (MII) is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Permissions beyond the scope of this license may be available at



Adjie S, Husnah, Gaffar AK. 1999. Studi biologi ikan belida (Notopterus chitala) di daerah aliran Sungai Batanghari. Provinsi Jambi. Jurnal Penelitian Perikanan Indonesia, 1: 38-43.

Bofkin L, Goldman N. 2007. Variation in evolutionary processes at different codon positions. Molecular Biology and Evolution. 24(2): 513-521.

Hebert PDN, Cywinska A, Ball SL, deWard JR. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B, 270: 313-322.

Inger RF, Chin PK. 1962. The fresh-water fishes of North Borneo. Fieldiana Zoology, 45: 1-268.

Jusmaldi, Solihin DD, Affandi R, Rahardjo MF, Gustiano R. 2014. Kode batang DNA ikan lais genus Kryptopterus asal Sungai Mahakam Kalimantan Timur. Jurnal Iktiologi Indonesia, 14(3): 191-199.

Kottelat M, Whitten JA, Kartikasari N, Wiryoatmojo S. 1993. Freshwater fishes of Western Indonesia and Sulawesi. Periplus Edition and Emdi Project Indonesia. Jakarta

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33(7): 1870-1874.

Li WH, Graur D. 2000. Fundamental of Molecular Evolution. Second edition. Sinauer Associates Inc. Sunderland (US). 481 p.

Menteri LHK. 2018. Peraturan Lingkungan Hidup dan Kehutanan Republik Indonesia. Nomor: P.20/ MENLHK/SETJEN/KUM.1/6/2018 tentang jenis tumbuhan dan satwa yang dilindungi.

MUAWC. 2008. Blood Collection Guidelines. Monash University Animal Welfare Committee (MUAWC). 1-10.

Nugroho E, Dewi RRSPN. Subagja J. Priono B. 2019. Keragaman genetik dan karakter biometrik ikan belida (Chitala lopis. Bleeker 1851) budidaya asal Sungai Kampar. Riau. Jurnal Riset Akuakultur, 14(1): 1-8.

Page TJ, Hughes JM. 2010. Comparing the performance of multiple mitochondrial genes in the analysis of Australian freshwater fishes. Journal of Fish Biology, 77(9): 2093-2122.

Santoso L. 2009. Biologi reproduksi ikan belida (Chitala lopis) di Sungai Tulang Bawang. Lampung. Berkala Perikanan Terubuk, 31(1): 38-46.

Sarkar UK, Negi RS, Deepak PK. Lakra WS. and Paul SK. 2008. Biological parameters of the endangered fish Chitala chitala (Osteoglossiformes: Notopteridae) from some Indian Rivers. Fisheries Research, 90(1): 170-177.

Sengupta S, Homechaudhuri S. 2013. DNA barcodes of some threatened freshwater indigenous fishes in India. Current Science, 105(1): 84-90.

Solihin DD. 1994. Peran DNA mitokondria (mtDNA) dalam studi keragaman genetik dan biologi populasi pada hewan. Hayati, 1(1):1-4.

Sudarto, Pouyaud L, Kusuma RV. 2008. Struktur populasi dan sejarah kolonisasi ikan botia (Chromobotia macracanthus Bleeker) asal Sumatera dan Kalimantan berdasarkan sekuen intron dari Gen Aldolase-B. Jurnal Perikanan, 9(2): 203-212.

Sudarto, Rizal M. 2007. Variasi morfometri ikan botia (Botia macracanthus Bleeker) dari perairan Sumatera dan Kalimantan. Jurnal Perikanan, 9(2): 214-219.

Sunarno MTD, Syamsunarno MB. 2015. Pengaruh naungan terhadap pematangan gonad dan pemijahan ikan belida (Chitala lopis) di kolam rawa. Jurnal Ilmu Pertanian dan Perikanan, 4(1): 71-77.

Sunarno MTD, Wibowo A, Subagja. 2007. Identifikasi tiga kelompok ikan belida (Chitala lopis) di Sungai Tulang Bawang. Kampar. dan Kapuas dengan pendekatan biometrik. Jurnal Penelitian Perikanan Indonesia, 13(2): 87-94.

Voris HK. 2000. Maps of pleistocene sea-levels in South East Asia: shorelines, river systems, time durations. Journal of Biogeography, 27(5): 1153-1167.

Ward RD, Hanner R, Hebert PDN. 2009. The campaign to DNA barcode all fishes, FISH-BOL. Journal of Fish Biology, 74(2): 329-356.

Ward RD, Holmes BH. 2007. An analysis of nucleotide and amino acid variability in the barcode region of cytochrome c oxidase I (cox1) in fishes. Molecular Ecology Notes, 7(6): 899-907.

Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B, 360: 1887-1857.

Wibowo A Subagja. 2014. Penilaian indeks kualitas lingkungan untuk menentukan wilayah konservasi ikan belida (Chitala lopis) di Sungai Kampar. Riau. Bawal, 6(1): 1-9.

Wibowo A, 2014. Barcoding ikan belida (Chitala Lopis) berdasarkan gen Cytochrome Oxidase Subunit I (COI) DNA mitokondria. In: Alim Isnansetyo (editor). Prosiding Seminar Nasional Tahunan XI Hasil Penelitian Perikanan dan Kelautan Tahun 2014 Jilid II. Jurusan Perikanan dan Kelautan Fakultas Pertanian UGM. Yogyakarta, pp. 227-235.

Wibowo A, Affandi R, Soewardi K, Sudarto. 2010. Genetic differentiation of the kampar river's giant featherback (Chitala lopis Bleeker 1851) base on mitochondrial dna analysis. Indonesian Fisheries Research Journal, 16(2): 49-58.

Wibowo A, Marson. 2012. Fenomena plastisitas fenotipik ikan belida (Chitala lopis) di Sungai Kampar. Riau. Bawal, 4(3): 195-204.

Wibowo A. 2012. Struktur genetik populasi ikan belida (Chitala lopis. Bleeker 1851) di Waduk Kutopanjang. Bawal, 4(1): 53-58.