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RESEARCH ARTICLE
Maturational induction of grass carp, Ctenopharyngodon idella (Valenciennes, 1844) using hormone and Indigofera zollingeriana feed
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Abstract
Pregnant mare serum gonadotropin (PMSG) premix hormone and anti dopamine were used to accelerate maturation. Indigofera zollingeriana a legume plant that has high nutrition content and carotenoids whose has function in oocyte development. The aim of this research was to accelerate the maturation of grass carp (Ctenopharyngodon idella) using premix hormone and to replace the commercial feed with indigofera. The research was conducted in couple randomize design (CRD), using five treatments which were A (NaCl 0.5 ml kg-1 body weight + commercial feed), B (NaCl 0.5 ml kg-1 body weight + indigofera feed), C (premix hormone 0.5 ml kg-1 body weight + commercial feed), D (premix hormone 0.5 ml kg-1 body weight + indigofera feed) and E (premix hormone 0.5 ml kg-1 body weight in indigofera feed) and five fishes as individual replication. The result showed that 17P- estradiol concentration reached the highest level at 4-week on C treatment 1194.8 pgmL-1, while in E treatment 17P-estradiol concentrations was higher than the other treatments at 8-week. At the end of research period, the highest gonadosomatic index (GSI) was performed by E treatment. Addition of premix hormone on feed could increase the gonadal maturation up to perinucleus phase, while in control was still immature. These results indicated that premix hormone could induce gonadal maturity, indigofera could replace commercial feed as broodstock diet, and gonadal maturation through hormonal induction could be perform by giving more competitive feed.
Abstrak
Premiks hormon pregnant mare serum gonadotropin (PMSG) dan anti dopamin dapat mempercepat pematangan gonad pada ikan. Indigofera zollingeriana merupakan tumbuhan leguminosa yang memiliki nutrisi tinggi dan mengandung ka-rotenoid yang berfungsi untuk perkembangan oosit. Tujuan penelitian ini untuk mempercepat pematangan gonad meng-gunakan premiks hormon serta menggantikan pakan komersial dengan pakan indigofera pada ikan koan (Ctenopha-ryngodon idella). Penelitian menggunakan rancangan acak lengkap dengan lima perlakuan yaitu A (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan komersial), B (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan indigofera), C (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan komersial), D (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan indigofera), E (premiks hormon 0,5 ml kg-1 bobot tubuh dalam pakan indigofera) dan lima kali ulangan individu. Hasil penelitian me-nunjukkan bahwa estradiol-17p mencapai puncak pada minggu keempat pada perlakuan C yaitu sebesar 1194,8 pg ml-1 sedangkan pada perlakuan E konsentrasi estradiol-17p lebih tinggi daripada perlakuan lain pada minggu kedelapan. Pada akhir penelitian indeks kematangan gonad tertinggi diperoleh pada perlakuan E. Penambahan premiks hormon pada pakan dapat meningkatkan kematangan gonad hingga mencapai fase perinukleus sedangkan pada kontrol tidak berkem-bang. Hasil ini menunjukkan bahwa premiks hormon dapat menginduksi kematangan gonad, pakan indigofera dapat menggantikan pakan komersial sebagai pakan induk, dan induksi pematangan gonad secara hormonal dapat dilakukan dengan pemberian pakan yang lebih kompetitif.
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