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Maturational induction of grass carp, Ctenopharyngodon idella (Valenciennes, 1844) using hormone and Indigofera zollingeriana feed
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Abstract
Pregnant mare serum gonadotropin (PMSG) premix hormone and anti dopamine were used to accelerate maturation. Indigofera zollingeriana a legume plant that has high nutrition content and carotenoids whose has function in oocyte development. The aim of this research was to accelerate the maturation of grass carp (Ctenopharyngodon idella) using premix hormone and to replace the commercial feed with indigofera. The research was conducted in couple randomize design (CRD), using five treatments which were A (NaCl 0.5 ml kg-1 body weight + commercial feed), B (NaCl 0.5 ml kg-1 body weight + indigofera feed), C (premix hormone 0.5 ml kg-1 body weight + commercial feed), D (premix hormone 0.5 ml kg-1 body weight + indigofera feed) and E (premix hormone 0.5 ml kg-1 body weight in indigofera feed) and five fishes as individual replication. The result showed that 17P- estradiol concentration reached the highest level at 4-week on C treatment 1194.8 pgmL-1, while in E treatment 17P-estradiol concentrations was higher than the other treatments at 8-week. At the end of research period, the highest gonadosomatic index (GSI) was performed by E treatment. Addition of premix hormone on feed could increase the gonadal maturation up to perinucleus phase, while in control was still immature. These results indicated that premix hormone could induce gonadal maturity, indigofera could replace commercial feed as broodstock diet, and gonadal maturation through hormonal induction could be perform by giving more competitive feed.
Abstrak
Premiks hormon pregnant mare serum gonadotropin (PMSG) dan anti dopamin dapat mempercepat pematangan gonad pada ikan. Indigofera zollingeriana merupakan tumbuhan leguminosa yang memiliki nutrisi tinggi dan mengandung ka-rotenoid yang berfungsi untuk perkembangan oosit. Tujuan penelitian ini untuk mempercepat pematangan gonad meng-gunakan premiks hormon serta menggantikan pakan komersial dengan pakan indigofera pada ikan koan (Ctenopha-ryngodon idella). Penelitian menggunakan rancangan acak lengkap dengan lima perlakuan yaitu A (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan komersial), B (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan indigofera), C (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan komersial), D (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan indigofera), E (premiks hormon 0,5 ml kg-1 bobot tubuh dalam pakan indigofera) dan lima kali ulangan individu. Hasil penelitian me-nunjukkan bahwa estradiol-17p mencapai puncak pada minggu keempat pada perlakuan C yaitu sebesar 1194,8 pg ml-1 sedangkan pada perlakuan E konsentrasi estradiol-17p lebih tinggi daripada perlakuan lain pada minggu kedelapan. Pada akhir penelitian indeks kematangan gonad tertinggi diperoleh pada perlakuan E. Penambahan premiks hormon pada pakan dapat meningkatkan kematangan gonad hingga mencapai fase perinukleus sedangkan pada kontrol tidak berkem-bang. Hasil ini menunjukkan bahwa premiks hormon dapat menginduksi kematangan gonad, pakan indigofera dapat menggantikan pakan komersial sebagai pakan induk, dan induksi pematangan gonad secara hormonal dapat dilakukan dengan pemberian pakan yang lebih kompetitif.
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References
Abdullah L, Suharlina. 2010. Herbage yield and quality of two vegetative parts of indigo-fera at different times of first regrowth defoliation. Jurnal Media Peternakan, 33(1): 44-49.
Abdullah L, Tarigan A, Suharlina, Budhi D, Jo-vintry I, Apdini TA. 2012. Indigofera zo-llingeriana: A promising forage and shrubby legum crop for Indonesia. In: Astuti DA (editor). Proceeding of the 2nd International Seminar on Animal Industry. Fakultas Peternakan Institut Pertanian Bogor. pp 70-72.
Akbarillah T, Kaharuddin, Kususiyah. 2002. Kajian daun tepung indigofera sebagai suplemen pakan produksi dan kualitas telur. Laporan Penelitian. Lembaga Penelitian Universitas Bengkulu, Bengkulu. 6 hlm.
Akbarillah T, Kususiyah, Kaharuddin, Hidayat. 2008. Kajian tepung daun indigofera sebagai suplemen pakan terhadap produksi dan kualitas telur puyuh. Jurnal Peternakan Indonesia, 3(1): 20-23.
Akbarillah T, Kususiyah, Hidayat. 2010. Pengaruh penggunaan daun indigofera segar sebagai suplemen pakan terhadap produksi dan warna yolk itik. Jurnal Sains Peternakan Indonesia, 5(1): 27-33.
Anbazahan S M, Mari LSS, Yogeshwari G, Jagruthi C, Thirumurugan R, Arockiaraj J, Velanganni AAJ, Krishnamoorthy P, Balasundaram C, Harikrishnan R. 2014. Immune response and disease resistance of carotenoids supplementation diet in Cypri-nus carpio against Aeromonas hydrophila. Fish & Shellfish Immunology, 40(1): 9-13.
Biard C, Surai PF, Meller AP. 2005. Effect of carotenoid availability during laying on reproduction in the blue tit. Oecologia, 144(1): 32-44.
Cudmore B, Mandrak NE. 2004. Biological synopsis of grass carp (Ctenopharyngodon della). Canadian Manuscript Report of Fisheries and Aquatic Science, 2705: 44 p.
Darwisito S, M Zairin Jr, Sjafei DS, Manalu W, Sudrajat AO. 2008. Pemberian pakan mengandung vitamin E dan minyak ikan pada induk memperbaiki kualitas dan larva ikan nila (Orechromis niloticus). Jurnal Akua-kultur Indonesia, 7(1): 1-10.
Dufour S, Weltzien FA, Sebert ME, Lebelle N, Vidal B, Verier P, Pasqualini C. 2005. Dopaminergic inhibition of reproduction in teleost fishes ecophysiological and evolutionary implications. New York Academy ofSciences. 1040: 9-21.
Dufour S, Sebert ME, Weltzien FA, Rousseau K, Pasqualini C. 2010. Neuroendocrine control by dopamine of teleost reproduction. Journal of Fish Biology, 76: 29-160.
Ghazala R, Tabinda AB, Yasar A. 2011. Growth respone of juvenile grass carp (Ctenopha-ringodon idella) fed isocaloric diets with variable protein levels, The Journal of Animal and Plant Sciences, 21(4): 850856.
Isomura N, Yamauchi C, Takeuchi Y, Takemura A. 2013. Does dopamine block the spawning of the acroporid coral Acropora tenuis? Scientific Reports. 3: 2649.
Izquerdo MS, Fernandez-Palacios H, Tacon AGJ. 2001. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture, 197(1-4): 25-42.
Kobayashi D, Tanaka M, Fukada S, Nagahama Y. 1996. Steroidogenesis in the ovarian follicles of the medaka (Oryzias latipes) during vitellogenesis and oocyte maturation. Zoological Science, 13(6): 921927.
Kobayashi M, Sorensen PW, Stacey NE. 2002. Hormonal and pheromonal control of spawning behavior in the goldfish. Fish Physiology and Biochemistry, 26(1): 7184.
Kog N D, Yener A, Rikap Y. 2008. Ovary maturation stages and histological investigation of ovary of the zebrafish (Danio rerio). Brazilian Archives of Biology Technology, 5(3): 513-522.
Lee M-F, Jing-Duan H, Ching-Fong C. 2012. Development of ovarian tissue and female germ cells in the protandrous black porgy,
Acanthopagrus schlegeli (Perciformes, Sparidae). Zoological Studies, 47(3): 302316.
Lubzens E, Young G, Bobe J, Cerda J. 2010. Oogenesis in Teleosts: How fish eggs are formed. General and Comparative Endocrinology, 165(3): 367-389.
Mitchell AJ, Kelly AM. 2006. The public sector role in the establishment of grass carp in the United States. Fisheries, 31(3): 113121.
Moore WT, Ward DN. 1980. Pregnant mare serum gonadotropin rapid chromatographic procedures for the purification of intact hormone and isolation of subunit. Journal of Biological Chemistry, 17(4): 69286929.
Mylonas CC, Fostier A, Zanuy S. 2010. Brood-stock management and hormonal manipulation of fish reproduction. General and Comparative Endocrinology, 165(3): 516534.
Nagahama Y, Yamashita M. 2008. Regulation of oocyte maturation in fish. Development, Growth and Differentiation, 50 (Supple-ment s1): S195-S219.
Palupi R. 2015. Subtitusi protein bungkil kedelai dengan protein tepung pucuk Indigofera zollingeriana untuk menghasilkan telur fungsional tinggi antioksidan. Disertasi. Institut Pertanian Bogor. 91 hlm.
Podhorec P, Kouril J. 2009. Induction of final oocyte maturation in Cyprinidae fish by hypothalamic factor: a review. Veterina Medicana, 54(3): 97-110.
Putra WKA. 2013. Induksi belut sawah (Mono-pterus albus) secara hormonal. Tesis. In-stitut Pertanian Bogor. 42 hlm.
Rachman B. 2013. Manipulasi hormonal pada pematangan gonad ikan patin siam Pa-ngasianodon hypophthalmus. Tesis. Institut Pertanian Bogor. 47 hlm.
Reidel A, Boscolo WR, Feiden A, Romagosa E. 2010. The effect of diets with different levels protein of Rhamdia quelen stocked in cages. Aquaculture. 298: 354-359.
Rosety M, Miguel B, Luisa GDC, Amalia G, Carmen S. 1992. Biochemical parameters during reproduction of the toad fish, Halo-batrachus didactylus (Schneider, 1801). Science Marine, 56 (1): 87-94.
Sagi A, Rise M, Isam K, Araf S. 1995. Carote-noids and their derivates in organs of the maturing female crayfish Cherax quadry-carinatus. Biochemistry and Molecular, 112(2): 309-313.
Santos IN, Andrade CC, Santos AF, Santos LN, Araujo FG. 2005. Histologycal analysis of ovarian development of the characiform
Oligosarcus hepsetus (Cuvier, 1829) in a Brazilian reservoir. Brazilian Biology Journal, 65(1): 77-169.
Senthilkumaran B, Yoshikuni M, Nagahama Y. 2004. A shift in steroidegenesis in ovarian follicles prior to oocyte maturation. Molecular and Cellular Endocrinology, 215: 11-18.