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Authors

Abdul Zahri
Agus Oman Sudrajat
Muhammad Zairin Junior

Abstract

The objective of the experiment was to describe the influence of estradiol (E2) and methyltestosterone (MT) in combination with hCG and anti-dopamine (AD) on the percentage of female and quality of ovary development of Indonesian short-finned eel (Anguilla bicolor bicolor). Eel with 200±15 g of body weight in the yellow phase injected with a estradiol solution of 3mg mL-1 + hCG 2mg mL-1 + AD 10 mg mL-1 (hEA), MT 3 mg mL-1 + hCG 2 mg mL-1 + AD 10 mg mL-1 (hTA), estradiol 3 mg mL-1 + AD 10 mg mL-1 (EA), MT 3 mg mL-1 + AD 10 mg mL-1 (TA), AD 10 mg mL-1 (A) and 9 mg mL-1 NaCl control (F). Injection frequency was six times with a period of two weeks. After six weeks of experiment, EA and hTA combination hormone treatments were effective to increase the female percentage 72% and 66%, respectively. The best ovary development was found in the hTA treatment i.e. attain the late-vitellogenesis with GSI value of 4.80%.


Abstrak


Penelitian ini bertujuan untuk menjelaskan pengaruh estradiol (E2) dan metil testosteron (MT) yang dikombinasikan dengan hCG dan anti dopamin (AD) terhadap persentase jenis kelamin betina dan kualitas perkembangan ovari sidat (Anguilla bicolor bicolor). Sidat dengan bobot 200±15g pada faseyellow diinjeksi dengan larutan estradiol 3 mg mL-1 + hCG, 2 mg mL-1 + AD 10 mg mL-1 (hEA), MT 3 mg mL-1 + hCG 2 mg mL-1 + AD 10 mg mL-1 (hTA), estradiol 3 mg mL-1 + AD 10 mg mL-1 (EA), MT 3 mg mL-1 + AD 10 mg mL-1 (TA), AD 10 mg mL-1 (A), dan kontrol 9 mg mL-1 NaCl (F). Frekuensi injeksi sebanyak enam kali dengan periode dua minggu sekali. Pemberian hormon kombinasi dengan perlakuan hEA dan hTA ternyata efektif meningkatkan persentase jenis kelamin betina berturut-turut sebesar 72% dan 66% setelah enam minggu. Perkembangan gonad betina terbaik ditemukan pada perlakuan hTA yaitu mencapai fase vitellogenesis akhir dengan nilai GSI 4,80%

Keywords:
hormone; feminization; gonad growth; Indonesian short-finned eel;

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References

Adachi S, Ijiri S, Kazeto Y, Yamauchi K. 2003. Oogenesis in the Japanese eel, Anguilla ja-ponica. In: Aida K, Tsukamoto K, Yama-uchi K. (Eds.). Eel biology. Springer-Ver-lag. Tokyo. pp. 301-318.

Anton M. 2007a. Low-density lipoprotein (LDL) or lipovitellenin fraction. In: Huopalahti R, Lopez-Fandino R, Anton M, Schade R. (Eds.). Bioactive Egg Compounds. Spri-nger-Verlag Berlin. pp. 7-12.

Anton M. 2007b. High-density Lipoprotein (HDL) or Lipovitellin Fraction. In. Huopa-lahti R, Lopez-Fandino R, Anton M, Scha-de R. (Eds.). Bioactive egg compounds. Springer-Verlag Berlin. pp. 13-16.

Anton M, Castellani O, Guerin-Dubiard C. 2007. Phosvitin. In: Huopalahti R, Lopez-Fandino R, Anton M, Schade R. (Eds.). Bioactive Egg Compounds. Springer-Verlag Berlin. pp. 17-24.

Cerda-Reverter JM, Canosa LF. 2009. Neuroendocrine system of the fish brain. In: Farrell AP, Brauner CJ (Eds.). Fish Physiology, 28: Fish Neuroendocrinology. Academic Press. London. pp. 3-74.

Chai Y, Tosaka R, Abe T, Sago K, Sago Y, Ha-tanaka E, Ijiri S, Adachi S. 2010. The relationship between the development stage of oocytes in various seasons and the quality of the egg obtained by artificial maturation in the feminized Japanese eel Anguilla ja-ponica. Aquaculture Science 58(2): 269278.

Chu Yu-Wei, Han Yu-San, Wang Chia-Hui, You Chen-Feng, Tzeng Wann-Nian. 2006. The sex-ratio reversal of the Japanese eel Anguilla japonica in the Kaoping River of Taiwan: The effect of cultured eels and its implication. Aquaculture 261(4): 1230-1238

Costa DDM, Neto FF, Costa MDM, Morais RN, Garcia JRE, Esquivel BM, Ribeiro CAO. 2010. Vitellogenesis and other physiological responses induced by 17-p-estradiol in males of freswater fish Rhamdia quelen. Comparative Biochemistry and Physiology Part C 151(2): 248-257.

Durif CMF, van Ginneken V, Dufour S, Muller T, Elie P. 2009. Seasonal evolution and individual differences in silvering eels from different locations. In: van den Thillart G, Dufour S, Rankin JC (Eds.). Spawning migration of the European eel. Springer Science. pp. 13-38.

Endo T, Todo T, Lokman PM, Kudo H, Ijiri S, Adachi S, Yamauchi K. 2011. Androgens and very low density lipoprotein are essential for the growth of previtellogenic oocytes from Japanese Eel, Anguilla japonica in vitro. Biology of Reproduction 84(4): 816825.

Fernandino JI, Hattori RS, Acosta ODM, Struss-mann CA, Somoza GM. 2013. Environmental stress-induced testis differentiation: androgen as a by-product of cortisol inactivation. General and Comparative Endocrinology 192(1): 36-44.

Higuchi M, Celino FT, Miura C, Miura T. 2012. The synthesis and role of taurine in the eel spermatogenesis. Amino Acids 43(2): 773781.

Ijiri S, Tsukamoto K, Chow S, Kurogi H, Adachi S, Tanaka H. 2011. Controlled reproduction in the Japanese eel (Anguilla japonica), past and present. Aquaculture Europe 36(2): 1317.

Kagawa H, Kasuga Y, Adachi J, Nishi A, Hashi-moto H, Imaizumi H, Kaji S. 2009. Effects of continuous administration of human chorionic gonadotropin, salmon pituitary extract, and gonadotropin-releasing hormone using osmotic pumps on induction of sexual maturation in male Japanese eel, Anguilla japonica. Aquaculture 296(1): 117-122.

Kearney M, Jeffs A, Lee P. 2011. Development and early differentiation of male gonads in farmed New Zealand shortfin eel, Anguilla australis. New Zealand Natural Sciences 36(1): 33-44.

Le Mann F, Cerda J, Babin PJ. 2007. Ultrastruc-tural aspects of the ontogeny and differentiation of ray-finned fish ovarian follicles. In: Babin PJ, Cerda J, Lubzens E. (Eds.).

The fish oocyte: from basic studies to biotechnological applications. Springer. Dordrecht. pp. 1-37.

Lokman PM, George KAN, Divers SL, Algie M, Young G. 2007. 11-Ketotestosterone and IGF-I increase the size of previtellogenic oocytes from shortfinned eel, Anguilla australis, in vitro. Reproduction 133(5): 955967.

Matsubara H, Tanaka H, Nomura K, Kobayashi T, Murashita K, Kurokawa T, Unuma T, Kim SK, Lokman MP, Matsubara T, Kaga-wa H, Ohta H. 2008. Occurrence of spontaneously spermiating eels in captivity. Cybium 32(2) suppl.: 174-175.

Melia P, Bavacqua D, Crivelli AJ, Panvilli J, De Leo GA, Gatto M. 2006. Sex differentiation of the European eel in brackish and freshwater environment: Comparatif analysis. Journal of Fish Biology 69(4): 1228-1235.

Miura T, Miura C, Yamauchi K. 2003. Spermatogenesis in the Japanese eel, Anguilla ja-ponica. In: Aida K, Tsukamoto K, Yama-uchi K. (Eds.). Eel biology. Springer-Verlag Tokyo. pp. 319-330.

Miura C, Miura T. 2011. Analysis of spermatogenesis using an eel model. Aqua-BioSci-ence Monographs (ABSM) 4(4): 105-129.

O’Connell LA, Fontenot MR, Hofmann HA. 2013. Neurochemical profiling of dopaminergic neurons in the forebrain of a cichlid fish, Astatotilapia burtoni. Journal of Chemical Neuroanatomy 47(1):106-115

Palstra A, Curiel D, Fekkes M, de Bakker M, Szekely C, van Ginneken V, van den Thil-lart G. 2007. Swimming stimulates oocyte development in European eel. Aquaculture 270(3): 321-332.

Palstra AP, Van den Thillart GEEJM. 2010. Swimming physiology of European silver eels (Anguilla anguilla L.): Energetic costs and effects on sexual maturation and reproduction. Fish Physiology and Biochemistry 36(3): 297-322.

Piferrer F, Blazquez. 2006. Aromatase distribution and regulation in Fish. Review. Fish Physiology and Biochemistry 31(2):215-226.

Rousseau K, Aroua S, Schmitz M, Elie P, Dufour S. 2009. Silvering: metamorphosis or puberty? In: van den Thillart G, Dufour S, Rankin JC (Eds.). Spawning migration of the European eel. Springer Science. pp. 3963.

Setiawan AN, Wylie MJ, Forbes EL, Lokman PM. 2012. The Effects 11-ketotestosterone on occupation of downstream location and seawater in the New Zealand shortfinned eel, Anguilla australis. Zoological Science 29(1): 1-5.

Singh V, Singh PB, Srivastava S. 2009. Testosterone and estradiol-17p dependent phospoli-pid biosynthesis in ovariectomized cathfish, Heteropneustes fossilis. Journal of Environmental Biology 30(5): 63-640.

Sugeha HY, Jatmiko I, Muhammad S. 2009. Sexual development of the tropical short-finned eel Anguilla bicolor bicolor of the Segara Anakan Waters, Central Java, Indonesia. Journal of Fisheries Sciences 11(1): 87-99.

Taranger GL, Carrillo M, Schulz RW, Pascal Fontaine P, Zanuy S, Felip A, Weltzien FA, Dufour S, Karlsen O, Norberg B, An-dersson E, Hansen T. 2010. Control of puberty in farmed fish. General and Comparative Endocrinology 165(3): 483-515.

Tomkiewicz J, Kofoed TMN, Pedersen JS. 2011. Assessment of testis development during induced spermatogenesis in the European eel Anguilla anguilla. Marine and Coastal Fisheries: Dynamics, Management, and Ecosystem. Science 3(1): 106-118.

Uno T, Ishizuka M, Itakura T. 2012. Cytochrome P450 (CYP) in fish. Review. Environmental Toxicology and Pharmacology 34(1):1-13.

van Ginneken V, Durif C, Dufour S, Sbaihi M, Boot R, Noorlander K, Doornbos J, Murk AJ, van den Thillart G. 2007. Endocrine profiles during silvering of the European eel (Anguilla anguilla L.) living in saltwater. Animal Biology 57(4): 453-465.

Versonnen BJ, Goemans G, Belpaire C, Janssen CR. 2004. Vitellogenin content in European eel (Anguilla anguilla) in Flanders, Belgium. Environmental Pollution 128(3): 363-371.

Wang HP, Gao Z, Beres B, Ottobre J, Wallat G, Tiu L, Rapp D, O'Bryant P, Yao H. 2008. Effects of estradiol-17p on survival, growth performance, sex reversal and gonadal structure of bluegill sunfish Lepomis mac-rochirus. Aquaculture 285(2): 216-223.

Wang YS, Lou SW. 2007. Influence of exogenous gonadotropin sexual steroids on ovary development in Anguilla japonica. Journal of the Fisheries Society of Taiwan 34(3): 261-273.

Weltzien F-A, Sebert M-E, Vidal B, Pasqualini C, Dufour S. 2009. Dopamin inhibition of eel reproduction. In: van den Thillart G, Dufour S, Rankin JC (Eds.). Spawning migration of the European eel. Springer Science. pp. 279-307.

Wilson CA, Davies DC. 2007. The Control of sexual differentiation of the reproductive system and brain. Review. Reproduction 133(2): 331-359.

Yokouchi K, Sudo R, Kaifu K, Aoyama J, Tsukamoto K. 2009. Biological characteristic of silver-phase Japanese eels, Anguilla japonica, collected from Hamana Lake, Japan. Coastal Marine Science 33(1): 1-10.