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Effect of fasting in waters with different salinity on physiological responses of Asian swamp eel, Monopterus albus Zuiew 1793
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Abstract
This study aimed to evaluate the physiological responses of Asian swamp eel, Monopterus albus during the fasting period at different salinity levels. Fasting period was three days at four levels of salinity, i.e., 0 g L -1 , 3 g L - 1 , 6 g L - 1 , and 9 g L - 1 . The eels were stocked at a density of 200 kg m - 3 . Physiological parameters studied were blood plasma cortisol which was measured by radioimmunoassay method, blood plasma glucose and liver glycogen which were measured by spectrophotometer, blood plasma sodium which was analyzed by Atomic Absorption Spect rophotometer, and osmolality which was measured using osmometer. The results showed that salinity affected the physiological status of the eels. Fasting the eels could increase the blood plasma cortisol levels, but in at higher salinity levels the hormone was lower. Blood plasma sodium levels of the eels increased in line with higher salinity. Fasting the eels at salinity of 0 and 3 g L - 1 was increasing the glucose levels significantly compared to the eel at a salinity of 6 and 9 g L - 1 . In contrary, the liv er glycogen levels were statistically lower in salinity of 0 and 3 g L - 1 compared to the eels at a salinity of 6 and 9 g L - 1 . Fasting the fish for three days at all salinity treatments did not change the condition factor of the eels. The eels were exhibite d the best physiological performance during the three - days fasting period at a salinity of 9 g L - 1 .
Abstrak
Penelitian ini bertujuan untuk meng evaluasi respons fisiologis ikan belut Monopterus albus pada pemberokan dengan tingkat salinitas yang berbeda. Pemberokan dilakukan pada ikan belut yang berukuran panjang 26,64 ± 2,94 cm dan b o - bot 12,57±4,99 g , selama tiga hari dalam media air dengan perlakuan empat tingkat salinitas, yaitu 0 g L - 1 , 3 g L -1 , 6 g L - 1 , dan 9 g L - 1 . B iomassa belut adalah 1 kg dalam 5 L air atau pada densitas 200 kg m - 3 . Parameter fisiologis yang diu - kur meliputi kortisol plasma darah dengan metode radioimunoasai, glukosa plasma darah dan glikogen hati dengan spektrofotometer, natrium plasma darah diana lisis dengan Atomic Absorption Spectrophotometer , dan osmolalitas de - ngan menggunakan osmometer. Hasil penelitian menunjukkan bahwa p emberokan dan salinitas mengakibatkan peru - bahan kondisi fisiologis ikan belut. Pemberokan mengakibatkan k adar kortisol meningkat , n amun peningkatan salinitas dapat menekan kadar hormon tersebut. Kadar natrium plasma darah ikan belut mengalami peningkatan sejalan dengan semakin tingginya salinitas. Kadar glukosa belut yang diberok pada salinitas 0 dan 3 g L - 1 secara statistik lebih tinggi dan berbeda sangat nyata di b andingkan dengan belut pada salinitas 6 dan 9 g L - 1 . Hal sebaliknya t erjadi pada kadar gli - kogen hati, yaitu secara statistik lebih rendah pada salinitas 0 dan 3 g L - 1 dibandingkan dengan belut pada salinitas 6 dan 9 g L - 1 . P emberokan selama tiga hari pada semua perlakuan salinitas t idak mengakibatkan perubahan nilai faktor kondisi belut . Ikan belut memberikan respons fisiologis yang paling baik pada pemberokan tiga hari dengan salinitas 9 g L - 1.
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References
 Affandi R, Ernawati Y, Wahyudi S. 2003. Studi bioekologi belut Monopterus albus pada berbagai ketinggian tempat di Kabupaten Subang Jawa Barat. Jurnal Iktiologi Indonesia , 3(2): 49 - 55
Alit IGK. 2009. Pengaruh padat penebaran terhadap pertambahan berat dan panjang badan belut (M onopterus albus ). Jurnal Biologi , 8 ( 1): 25 - 28 .
Ashley P J. 2007. Fish welfare: current issues in aquaculture. Applied Animal Behaviour Science , 104(3): 199 - 235.
Barman J, Baruah UK, Goswami UC. 2013. Indigenous techniques of catching the mud eel, Monopterus cuchia (Ham.) in Goalpara district, Assam. Indian Journal of Traditional Knowledge , 12(1): 109 - 115
Barton BA. 2002. Stress in Fishes: A diversity of responses with particular reference to changes in circulating corticosteroids. I nteg rated Comp arative Biol ogy , 42 (3): 517 - 525.
Berka R. 1986. The transport of live fish. A re - view . EIFAC Technical Paper 48 : 52 p.
Carneiro PSF, Swarofsky EC, Souza D PE, César TMR, Baglioli B, Baldisserotto B. 2009. Ammonia - , sodium chloride - , and calcium sulfate - induced changes in the stress responses of jundiá, Rhamdia quelen , juveniles. Journal of the World Aquaculture Society , 40(6): 810 – 817.
Cole RA, Choudhury A, Nico LG, Griffin KM. 2014. Gnathostoma spinigerum in live Asian swamp eels Monopterus spp. from food markets and wild populations, United States. Emerg ing Infect ious Dis eases , 20(4): 634 – 642.
Febry R, Lutz P. 1987. Energy partitioning in fish: the activity - related cost of osmoregu - lation in a euryhaline cichlid. J ournal of Exp erimental Bio logy , 128(1): 63 - 85
Fujiani T, Efrizal, Rahayu R. 2015. Laju pertum - buhan belut sawah ( Monopterus albus Zuiew) dengan pemberian berbagai pakan. Jurnal Biologi Universitas Andalas 4(1): 50 - 56.
Gomes LC, Araujo - Lima CARM, Roubach R, Chippari - Gomes AR, Lopes NP, Urbinati EC. 2006. Effect of fish density during transportation on stress and mortality of juvenile ta m baqui Colossoma macropomum . Journal of the World Aqua c u lture Society , 34(1): 493 - 502
Hogstrand C. Ferguson EA, Galvez F, Shaw JR , Webb NA, Wood CM. (1999). Physiology of acute silver toxicity in the starry flounder ( Platichthys stellatus ) in seawater. Journal of Comparative Physiology B , 169 (7): 461 - 573.
Ishim a tsu A. 2012. Evolution of the cardiorespiratory system in air - breathing fishes. Aqua - Bioscience Monographs , 5(1): 1 - 2 8.
Khan NH, Ngan HTB. 2010. Current practices of rice field eel Monopterus albus (Zuiew, 1793) culture in Viet Nam. Aquaculture Asia Magazine, 15(3) : 26 - 29
Kirsch R. 1972. Plasma chloride and sodium and chloride space in the European eel Anguilaanguilla L. J ournal of Exp erimental Biology , 57 (1) : 113 - 131
Koakoski G, Kreutz LC, Fagundes M, Oliveira TA, Ferreira D, da Rosa JGS, Barcellos LJG. 201 3 . Repeated stressors do not provoke habituation or accumulation of the stress response in the catfish Rhamdia quelen . Neotropical Ichthyology , 11(2): 453 - 457.
Koe ypudsa W, Jongjareanjai M. 2011. Impact of water temperature and sodium chloride on stress indicator of hybrid catfish (Clarias gariepinus Burchell x C. macrocephalus Gunther). Songklanakarin J ournal of Sci ence and Technol ogy, 33(4): 369 - 378
Kostecki PT. 1984. The effect of osmotic and ion - osmotic stresses in the blood and urine composition and urine flow of rainbow trout ( Salmo gairdneri ). C om parative Biochemistry and Physio logy , 79A(2): 215 - 221
Kumar P, Saurabh S, Pak AK, Sahu NP, Arasu AR. 2014. Stress mitigating and growth enhancing effect of dietary tryptophan in rohu ( Labeo rohita, Hamilton, 1822) fingerlings. Fish Physiol ogy and Biochemistry , 40(5): 1325 - 13 38.
Kultz D. 2015. Review. Physiological mechanisms used by fish to cope with salinity stress. The Journal of Experimental Biology , 218 (12): 1907 - 1914
Laiz - Carrión R, Martín del Río MP, Miguez JM, Mancera JM, Soengas JL. 2003. Influence of cortisol on osmoregulation and energy metabolism in gilthead sea bream Sparus aurata. Journal of Experimental Zoology Part A: Comparative Experimental Biology , 298 (2): 105 - 118 .
Liem KF.1967. Functional morphology of the integumentary, respiratory, and dig estive systems of Synbranchoid f ish Monopterus albus. Copeia , 2: 375 - 388.
Lisboa V, Barcarolli IF, Sampaio LA, Bianchini A. 2015. Effects of salinity on survival, growth and bichemical parameters in ju - venile Lebranch mullet Mugil liza (Perciformes: Mugilidae). Neotropical Ichthyology, 13(2): 447 - 452
Mehr ani H, Storey KB. 1993. Control of glycol - genolysis and effects of exercise on phosphorylase kinase and cAMP - dependent protein kinase in rainbow trout organs. Biochemistry and Cell Biology , 71(11 - 12): 501 - 506
Nash RDM, Valencia AH, Geffen AJ. 2006. The origin of Fulton’s condition factor - setting the record straight. Fisheries , 31 (5) : 236 - 239
Nico LG. 2005. Monopterus albus. USGS (U.S. Geological Survey) Nonindigenous Aquatic Species Database, Gainesville, Florida. Available: http://nas.er.usgs.gov/queries/ FactSheet. asp?species ID¼ 974 (July 2005)
Nico LG, Sharp P, Collins TM. 2011. Imported Asian swamp eels (Synbranchidae: Monopterus ) in North American live food markets: Potential vectors of non - native para - sites. REABIC Aqu atic Invasions, 6(1): 69 – 76.
Nikoo M, Falahatkar B, Alekhorshid M, Haghi BN, Asadollahpour A, Dangsareki MZ,
Langroudi HF. 2010. Physiological stress responses in kutum Rutilus frisii kutum subjected to captivity. International Aquatic Research, 2(1): 55 - 60
Oyoo - Okoth E, Cherop L, Ngugi CC, Chepkiruo Boit V, Manguya - Lusega D, Ani - Sabwa J, Kharo - Karisa H. 2011. Survival and physiological response of Labeo victorianus (Pisces: Cyprinidae, Boulenger 1901) juvenile to transport stress unde a salinity gradi ent. Aquaculture 319 (1): 226 - 231
Pedersen PBM, Hansen K, Houng DTT, Bayley M, Wang T. 2012. Effects of salinity on osmoregulation, growth and survival in Asian swamp eel ( Monopterus a lbus ) (Zuiew 1793). Aquaculture Research, 45 (3) : 427 – 438.
Pickering AD. 1981. Stress and Fish . Academic Press. London . 367 p .
Rainboth WJ. 1996. Fishes of the Cambodian Mekong . FAO S pecies I dentification Field G uide for Fi shery P urposes. M - 40 ISBN 92 - 5 - 103743 - 4. FAO, Rome, Italy. 265 p
Reid SG, Bernier NJ, Perry SF. 1998. Review. The adrenergic stress response in fish: control of catecholamine storage and release. Comparative Biochemistry and Physiology , Part C 120 (1) : 1 – 27
Sarma K, Prabakaran K, Khrisnan P, Grinson G, Kumar AA. 201 3 . Response of air breathing fish, Clarias batrachus to salinity stres s: an experiment case for their farming in brackishwater areas in Andaman, India. Aquaculture Int ernational , 21(1): 183 - 196.
Schofield PJ, Nico LJ. 2009. Salinity tolerance of non - native Asian swamp eels (Teleostei: Synbranchidae) in Florida, USA: comparison of three populations and implications for dispersal. Environ mental Biol ogy of Fish, 85 (1) : 51 – 59.
Shafland PL, Gestrin g KB, Stanford MS. 2010. An a ssessment of the Asian swamp eel ( Monopterus albus ) in Florida. Reviews in Fisheries Science, 18(1): 25 - 39
Specker JL, Schreck CB. 1980. Stress response to transportation and fitness for marine survival in coho salmon ( Oncorhynchus kisutch ) smolts. Canadian Journal of Fisheries and Aquatic Science, 37(5): 765 - 769.
Tay ASL, Chew S, Ip YK . 2003. The swamp eel Monopterus albus reduces endogenous ammonia production and detoxifies ammonia to glutamine during 144 h of aerial exposure. The J ournal of Exp erimental Biology, 206 (Pt 14) : 2473 - 2486.
Tok CY, Chew SF, Pek WYZ, Loong AM, Wong WP, Ip YK. 2009. Glutamine accumulation and up - regulation of glutamine synthetase activity in the swamp eel Monopterus albus (Zuiew) exposed to brackishwater. The J ournal of Exp erimental Biology, 212 (Pt 9) : 1248 - 1258
Tok CY, Chew SF, Ip YK. 2011. Gene cloning and mRNA expression of glutamate dehydrogenase in liver, brain, and intestine of the swamp eel, Monopterus albu s (Zuiew), exposed to fresh water, terrestial condition, environmental ammonia or salinity stress. Frontiers in Physiology , 2 (100): 1 - 11.
Tsuzuki MY, Ogawa K, Strussmann CA, Maita M, Takashima F. 2001. Physiological responses during stress and subsequent recovery at differe nt salinities in adult pejerrey Odontesthes bonariensis. Aquaculture , 200 (3) : 349 - 362
Urbinati CE, Carneiro PCF. 2006. Sodium chloride added to transport water and physiological responses of Matrinxã Brycon amazonicus (Teleost: Characidae). Acta Amazonic a, 36(4): 569 - 572
Wedemeyer GA. 1996. Physiology of Fish in Intensive Culture Systems. Chapman & Hall New York. 232 p.
Wurts WA. Using salt to reduce handling stress in channel catfish. World Aquaculture, 37(3): 26 - 27.

 
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