The evaluation of turmeric (Curcuma longa) supplementation within feed as an antioxidant towards growth performance of catfish Clarias gariepinus Burchell 1822 in zero water exchange condition

Thoy Batun Citra Rahmadani, Dedi Jusadi, Mia Setiawati, Yuni Puji Hastuti


The objective of this research was to evaluate the supplementation of turmeric in the diet on antioxidant status and growth performance of African catfish (Clarias gariepinus) in zero water exchange condition. This study used a completely randomized design with 4 treatments and 4 replications. Each treatment consisted of feed supplementation turmeric at dosage of 0; 2.5; 5 or 7.5 g kg-1 diet. One hundred catfish juvenile (5.95±0.05 g) were stocked in intermediate bulk container (IBC) tank (1×1×1 m3) and rearing in zero water exchange condition for 60 days. Catfish were fed at satiation twice a day, in the morning and evening. The results showed that an increase in antioxidant content in catfish fed with the addition of turmeric, which simultaneously also reduced the percentage of liver damage. The parameters of liver damage can be seen from several parameters i.e. pale liver, droplet fat and fat content in the addition of turmeric treatment is lower than without the addition of turmeric. However, catfish fed with the addition of turmeric did not show significant results in terms of growth performances.


antioxidant; catfish, turmeric; zero water exchange

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Ali TM, Esawy BHE, Elmorsy EA. 2015. Effect of combining an angiotensin enzyme inhibitor and a vitamin D receptor activator on renal oxidative and nitrosative stress in diabetic rats. National Journal of Physiology, Pharmacy and Pharmacology, 5(3): 222-231.

Alrawaiq NS, Abdullah A. 2014. A review of antioxidant polyphenol curcumin and its role in detoxification. Review. International Journal of Pharmacy and Technology Research, 6(1): 280-289.

Arisa AA. 2019. Peran monosodium glutamat dalam pakan pada produksi ikan lele Clarias gariepinus yang dibudidayakan pada air mengalir dan air tergenang. Tesis. Sekolah Pascasarjana. Institut Pertanian Bogor. Bogor.

Association of Official Analytical Chemist (AOAC). 2019. Official Method of Analysis. 21st Edition. Washington D.C

Benly ACK., Köksal G., Özkul A. 2008. Sublethal ammonia exposure of Nile Tilapia (Oreochromis niloticus L.): effects on gill, liver and kidney histology. Chemosphere, 72(9): 1355–1358.

Camargo JA, Álonso A. 2006. Ecological and toxicological effects of inorganic nitrogen pollution in aquatic ecosystems: a global assessment. Environmental International, 32(6): 831–849.

Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. 2004. Turmeric and curcumin: biological actions and medicinal applications. Current Science, 87(1): 44-53.

Chen S, Yu Y, Gao Y, Yin P, Tian L, Niu J, Liu Y. 2019. Exposure to acute ammnonia stress influences survival, immune response and antioxidant status of pacific white shrimp (Litopenaeues vannamei) pretreated with diverse levels of inositol. Fish and Shellfish Immunology, 89: 248-256.

Daneshyar M, Ghandkanlo MA, Bayeghra FS, Farhangpajhoh, Aghaei M, 2011. Effects of dietary turmeric supplementation on plasma lipoproteins, meat quality and fatty acid composition in broilers. South African Journal of Animal Science, 41(4): 420- 428

Dewi CD, Ekastuti DR, Sudrajat AO, Manalu W. 2017. Imroved vitellogenesis, gonad development and egg diameter in catfish (Pangasianodan hypophthalamus) supple-mented with turmeric (Curcuma longa) powder. Aquaculture Research, 49(2): 651-658.

Dyk JCV, Cochrane MJ, Wagenaar GM. 2012. Liver histopathology of the sharptooth catfish Clarias gariepinus as a biomarker of aquatic pollution. Chemosphere, 87(4): 301-311.

El-Beltagi H, Mohamed H. 2013. Reactive oxygen species, lipid peroxidation and antioxidative defense mechanism. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41(1): 44-57.

Foss A, Imsland AK, Roth B, Schram E, Stefansson SO. 2009. Effects of chronic and periodic exposure to ammonia on growth and blood physiology in juvenile turbot (Scophthalmus maximus). Aqua-culture, 296(1-2): 45-50.

Geijer A, Kim SK, Gerloff T, Dietrich CG, Lammert F, Karpen SJ. 2002. Hepatobiliary organic anion transporters are differentially regulated in acute toxicliver injury induced by carbon tetrachloride. Journal of Hepatology, 37(2) 198-205.

Giri SS, Sukumaran V, Park SC. 2011. Effect of bioactive substance from turmeric on growth, skin mucosal immunity and antioxidant factors in common carp, Cyprinus carpio. Fish and Shellfish Immunology, 92: 612-620.

Gluchowski NL, Becuwe M, Walther TC, Farese RV. 2017. Lipid droplets and liver disease: from basic biology to clinical implications. A review. Nature Reviews Gastroenterology & Hepatology, 14(6): 343-355.

Guan B, Hu W, Zhang T, Duan M, Li DL, Wang Y, Zhu Z. 2010. Acute and chronic un-ionized ammonia toxicity to ‘all-fish’ growth hormone transgenic common carp (Cyprinus carpio L.). Chinese Science Bulletin, 55(35): 4032-4036.

Hopkins KD. 1992. Reporting fish growth; a review of the basics. Journal of the World Aquaculture Society, 23(3): 173-179.

Jin Y, Tan Y, Chen L, Liu Y, Ren Z. 2018. Reactive oxygen species induces lipid droplet accumulation in HepG2 cells by increasing perilipin 2 expression. Inter-national Journal of Molecular Sciences, 19(11): 3445

Kocaadam, B, Şanlier, N. 2015. Curcumin, an active component of turmeric (Curcuma longa), and its effects on health. Critical Reviews in Food Science and Nutrition, 57(13): 2889–2895.

Li C, Zhang M, Li M, Zhang Q, Qian Y, Wang R. 2018. Effect of dietary alanyl-glutamine dipeptide against chronic ammonia stress induced hyperammonemia in the juvenile yellow catfish (Pelteobagrus fulvidraco). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 213: 55-61.

Mahmoud MMA, El-Lamie MMM, Dessouki AA, Yusuf MS. 2014. Effect of turmeric (Curcuma longa) supplementation on growth performance, feed utilization, and resistance of nile tilapia (Oreochromis niloticus) to Pseudomonas fluorescens challenge. Global Research Journal of Fishery Science and Aquaculture, 1(12): 26-33.

Murthy CRK, Rama Rao KV, Bai G, Norenberg MD. 2001. Ammonia-induced production of free radicals in primary cultures of rat astrocytes. Journal of Neuroscience Research, 66(2): 282-288

Ngaddi A. 2019. Evaluasi penggunaan monosodium glutamate terhadap respons fisiologis, kinerja pertumbuhan, dan pe-manfaatan pakan pada ikan lele Clarias gariepinus. Tesis. Sekolah Pascasarjana Institut Pertanian Bogor.

Saha N, Dutta S, Bhattacharjee A. 2002. Role of amino acid metabolism in an air-breathing catfish, Clarias batrachus in response to exposure to a high concentration of exogenous ammonia. Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, 133(2): 235-250.

Schram E, Roques JAC, Abbink W, Spanings T, de Vries P, Bierman S, Vis HVD, Flik G. 2010. The impact of elevated water ammonia concentration on physiology, growth and feed intake of African catfish (Clarias gariepinus). Aquaculture, 306(1): 108-115.

Shukla PK, Khanna VK, Khan MY, Srimal RC. 2003. Protective effect of curcumin against lead neurotoxicity in rat. Human & Experimental Toxicology, 22(12): 653-668.

Singh H, Bedi PS, Singh B. 2011. Hepatoprotective activity of turmeric and garlic against 7-12, dimethylbenzan-thracene induced liver damage in wistar albino rats. European Journal of Medicinal Plants, 1(4): 162-170.

Slaoui M, Fiette L. 2011. Histopathology procedures: from tissue sampling to histopathological evaluation. Methods in Molecular Biology, 691: 69-82.

Sun H, Lu K, Minter EJA, Chen YY, Montages D. 2012. Combined effects of ammonia and microcystin on survival, growth, anti-oxidant responses, and lipid peroxidation of bighead carp Hypophthalmythys nobilis larvae. Journal of Hazardous Materials, 221-222: 213-219

Tayyem RF, Hetah DD, Al-Delaimy KA, Rock CL. 2006. Curcumin content of turmeric and curry powders. Nutrition and Cancer, 55(2): 126-131.

Tung BT, Nham DT, Hai NT, Thu DK. 2019. Curcuma longa, the polyphenolic curcumin compound and pharmacological effects on liver (Chapter 3). Dietary Interventions in Liver Disease: Foods, Nutrients and Dietary Supplements. Academic Press. 428 p.

Wang HJ, Xu-Cheng X, Hong-ZhuW, Yan L, Qing Y, Xiao-Min L,Wei-Song F, Jian-Chun S, Marcus D, Erik J. 2017. Effects of high ammonia concentrations on three cyprinid fish: Acute andwhole-ecosy stem chronic tests. Science of Total Environment, 598: 900-909.

Watanabe T. 1988. Fish Nutrition and Mariculture. Departement of Aquatic Bioscience, Tokyo University, Tokyo.

Yarru LP, Settivari RS, Gowda NKS, Antoniou E, Ledoux DR, Rottinghaus GE. 2009. Effects of turmeric (Curcuma longa) on the expression of hepatic genes associated with biotransformation, antioxidant, and immune system in broiler chicks fed aflatoxin. Poultry Science, 88(12): 2620-2627.

Zhang W, Silei X, Zhu J, Miao X, Ren M, Lin Y, Ge X, Sun S. 2018. Growth performance, physiological response and histology changes of juvenile blunt snout bream, Megalobrama amblycephala exposed to chronic ammonia. Aquaculture, 506: 424-436.

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