Effectivity of Streptococcus agalactiae bacterial vaccine with different coatings for increasing the immunity system on nile tilapia Oreochromis niloticus (Linnaeus, 1758)


Ricko Reynalta, Munti Yuhana, Angela Mariana Lusiastuti
 DOI  https://doi.org/10.32491/jii.v19i2.478

Abstract

Vaccination is one of the most effective control measure in preventing Streptococcus agalactiae infection. In this study, vaccine coatings were prepared with certain biomaterials such as chitosan, skim milk, and maltodextrin at concentration 1 % or 10 %, and further freeze-dried. Vaccine selected (in vitro) by tests: viability cell, solubility, protein concentration and protein molecular weight. The result of in vitro test showed that chitosan coating at doses 1 % and 10 % were the best in solubility and protein concentration test. Vaccine coated with chitosan was administrated again by injection (in vivo) in nile tilapia (Oreochromis niloticus) before challenged by S. agalactiae. This test consists of six treatments and three replications, i.e. the fish were injected with liquid vaccine (A), Phospat Buffered Saline (PBS) (B), vaccine with chitosan coating 1 % (C), vaccine with chitosan coating 10 % (D), chitosan 1 % (E), and chitosan 10 % (F). Parameters were observed including survival rate, relative percent survival (RPS), antibody titre, and total leucocyte. The results showed that treatment C showed a significant difference (P < 0,05) in survival rate 92.22 ± 3.85 %, RPS 85.21 ± 7.20 %, and antibody titre highest result, but not significant (P > 0,05) to increase total leukocyte in experimental fish against S. agalactiae infection. In conclusion, vaccine freeze dry with chitosan coated 1 % is effective to improve immunity system of nile tilapia.

Keywords

chitosan; coating; Oreochromis niloticus; Streptococcus agalactiae; vaccine

Full Text:

PDF

References

Amal MNA, Zaad MZ. 2011. Streptococcosis in tilapia (Oreochromis niloticus). Pertanika Journal of Tropical Agricultural Science, 34(2): 195–206.

Biller JDT, Montassier HJ, Takahashi LS, Urbinati EC. 2014. Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus. Brazi-lian Journal of Biology, 74(1): 238-242.

Blaxhall PC, Daisley KW. 1973. Routine haematolo-gycal methods for use with fish blood. Journal of Fish Biology, 5(6): 577-581.

Bollag MD, Edelstein SJ. 1991. Protein Methods. Wiley-Liss. New York. 170 p.

Cock LS, Castillo VV. 2013. Probiotic encapsulation. African Journal of Microbiology Research, 7(40): 4743-4753.

Dwinanti SH, Sukenda, Yuhana M, Lusiastuti AM. 2014. Toksisitas dan imunogenitas produk ekstraseluler Streptococcus agalactiae tipe non-hemolitik pada ikan nila (Oreochromis niloticus). Jurnal Akuakultur Rawa Indonesia, 2(1): 105-116.

Effendie MI. 1997. Biologi Perikanan. Yayasan Pus-taka Nusatama. Yogyakarta. 157 p.

Ellis AE. 1988. General principles of fish vaccina-tion. In: Ellis AE (ed.). Fish Vaccination. Academic Press, London. pp. 1-19.

Estiasih T. 2012. Adsorpsi kompetitif fosfolipid pada permukaan globula minyak dalam sistem emulsi yang distabilisasi kaseinat. Jurnal Teknologi Pertanian, 13(1): 16-26.

Hartika R, Mustahal, Putra AN. 2014. Gambaran da-rah ikan nila (Oreochromis niloticus) dengan penambahan dosis prebiotik yang berbeda da-lam pakan. Jurnal Perikanan dan Kelautan, 4(4): 259-267.

Husniati. 2009. Studi karakterisasi sifat fungi malto-dekstrin dari pati singkong. Jurnal Riset Industri, 3(2): 133-138.

Irianto HE, Muljanah I. 2011. Proses dan aplikasi nanopartikel kitosan sebagai penghantar obat. Squalen, 6(1): 1-8.

Kanmani P, Kumar RS, Yuvaraj N, Paari KA, Pattu-kumar V, Arul V. 2011. Cryopreservation and microencapsulation of a probiotic in alginate kitosan capsules improves survival in simula-ted gastrointestinal conditions. Biotechnology and Bioprocess Engineering, 16(6): 1106-1114.

[KKP] Kementerian Kelautan dan Perikanan. 2017. Dashboard Produksi Perikanan dan Kelautan 2017 [internet]. [diunduh 17 November 2018]. Tersedia pada: https://satudata.kkp.go.id/ dashboard_produksi.

Kumru OS, Joshi SB, Smith DE, Middaugh CR, Prusik T, Volkin DB. 2014. Vaccine instability in the cold chain: Mechanisms, analysis and formulation strategies. Biologicals, 42(5): 237-259.

Kurniasih M, Kartika D. 2011. Sintesis dan karak-terisasi fisika-kimia kitosan. Jurnal Inovasi, 5(1): 42-48.

Lusiastuti AM, Purwaningsih U, Sumiati T. 2010. Isolasi bakteriofaga anti Streptococcus aga-lactiae dari ikan nila (Oreochromis niloticus). Jurnal Riset Akuakultur, 5(2): 237-243.

Matofani AS, Hastuti S, Basuki F. 2013. Profil darah ikan kunti (Oreochromis niloticus) yang di-injeksi Streptococcus agalactiae dengan kepadatan berbeda. Journal of Aquaculture Management and Technology, 2(2): 64-72.

Nahariah, Legowo AM, Abustam E, Hintono A. 2015. Aktivitas antioksidan dan antihipertensi tepung putih telur hasil ”Pan Drying” pada suhu dan waktu pengeringan yang berbeda. Jurnal Ilmu dan Teknologi Peternakan, 4(1): 28-34.

Pentury MH, Nursyam H, Harahap N, Soemarno. 2013. Karakterisasi maltodekstrin dari pati hipokotil mangrove (Bruguiera gymnorrhiza) menggunakan beberapa metode hidrolisis enzim. Indonesian Green Technology Journal, 2(1): 53-60.

Roberson BS. 1990. Bacterial aglutination. In: Stolen JS, Fletcher TC, Danerson DP, Roberson BS, van Muiswinkel WB. Techniques in Fish Immunology. SOS Publications. New Haven (US). pp.81-86.

Sari AN, Moeljaningsih. 2011. Pengaruh penambah-an susu skim dan konsentrasi starter (Lactoba-cillus casei) dalam pembuatan es krim susu jagung probiotik. Berita Litbang Industri, 16(1): 36-46.

Sukenda, Febriansyah TR, Nuryati S. 2014. Efikasi vaksin sel utuh Streptococcus agalactiae pada ikan nila Oreochromis sp. melalui perendam-an. Jurnal Akuakultur Indonesia, 13(1):83-93.

Sukenda, Rusli, Nuryati S, Hidayatullah D. 2015. Durasi proteksi vaksin Streptococcus agalac-tiae untuk pencegahan streptococcocis pada ikan nila. Jurnal Akuakultur Indonesia, 14(2): 192–201.

Sugiani D, Sukenda, Harris E, Lusiastuti AM. 2013. Vaksinasi ikan tilapia (Oreochromis niloticus) menggunakan vaksin monova-len dan bivalen untuk pencegahan penyakit Motile Aeromonas Septicemia dan Streptococcocis. Jurnal Riset Akuakultur, 8(2): 230-239.

Taukhid, Lusiastuti AM, Sumiati T. 2014. Apli-kasi vaksin Streptococcus agalactiae untuk pencegahan penyakit streptococco-sis pada budidaya ikan nila (Oreochromis niloticus). Berita Biologi, 13(3): 245-253.

Taukhid, Purwaningsih U. 2011. Penapisan isolat bakteri Streptococcus spp. sebagai kandi-dat antigen dalam pembuatan vaksin, serta efikasinya untuk pencegahan penyakit streptococcosis pada ikan nila, Oreochro-mis niloticus. Jurnal Riset Akuakultur, 6(1): 103-118.

Utami DAS, Widanarni, Suprayudi MA. 2015. Administration of microencapsulated pro-biotic at different doses to control strepto-coccosis in tilapia (Oreochromis niloti-cus). Microbiology Indonesia, 9(1): 17-24.

Wali A, Balkhi MUH. 2006. Fish vaccination and therapeutics. International Journal of Multidisciplinary Research and Develop-ment, 3(4): 55-60.

Article Metrics

 10.32491/jii.v19i2.478
   Abstract views: 22   PDF views or download: 10

 

Copyright (c) 2019 Jurnal Iktiologi Indonesia
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Refbacks

  • There are currently no refbacks.