[Hormone profile of FSH, LH and estradiol with glucose blood level of Indonesian short-finned eel (Anguilla bicolor bicolor Mc Clelland, 1844) stimulated by HCG, MT, E2 and dopamine inhibitory


Abdul Zahri, Agus Oman Sudrajat, Muhammad Zairin Junior
 DOI  https://doi.org/10.32491/jii.v18i1.374

Abstract

This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range.

 

Abstrak

This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range.


Keywords

glucose; hormone; gonad maturation; Indonesian short-finned eel

Full Text:

PDF

References

Aarestrup K, Thorstad EB, Koed A, Svendsen JC, Jepsen N, Pedersen MI, Økland F. 2010. Survival and progression rates of large European silver eel Anguilla anguilla in late freshwater and early marine phases. Aquatic Biology, 9(1): 263–270.

Aronoff SL, Berkowitz K, Shreiner B, Want L. 2004. Glucose metabolism and regulation: beyond insulin and glucagon. Diabetes Spectrum; 17, 3; pg. 183.

Aroua S, Maugars G, Jeng SR, Chang CF, Weltzien FA, Rousseau K, Dufour S. 2012. Pituitary gonadotropins FSH and LH are oppositely regulated by the activin/follistatin system in a basal teleost, the eel. General and Comparative Endocrinology, 175(1): 82–91.

Bartoňková J, Hyršl P, Vojtek L. 2016. Glucose determination in fish plasma by two different moderate methods. Acta Veterinární Brno. 85(1): 349–353.

Butts IAE, Sørensen SR, Politis SN, Pitcher TE, Tomkiewicz J. 2014. Standardization of fertilization protocols for the European eel, Anguilla anguilla. Aquaculture. 426–427: 9–13.

Cerda-Reverter JM, Canosa LF. 2009. Neuroendocrine System of the Fish Brain. In: Bernier NJ, Farrell AP, van der Krak G, Brauner CJ. (Eds.). Fish Physiology, 28: Fish Neuroendocrinology. Academic Press, London (UK). pp. 3–74.

Eames SC, Philipson LH, Prince VE, Kinkel MD. 2010. Blood sugar measurement in zebrafish reveals dynamics of glucose homeostasis. Zebrafish, 7(2): 205–213.

Fahmi MR, Solihin DD, Soewardi K, Pouyaud L, Shao Z, Berrebi P. 2013. A novel semi-multiplex PCR assay for identification of tropical eels of genus Anguilla in Indonesia waters. Fisheries Science. 79(2): 185–191.

Inoue JG, Miya M, Miller MJ, Sado T, Hanel R, Hatooka K, Aoyama J, Minegishi Y, Ni-shida M, Tsukamoto K. 2010. Deep-ocean origin of freshwater eels. Biology Letters, 6(3): 363–366.

Ishihara M, Abe T, Kazeto Y. Ijiri S, Adachi S. 2011. Effects of gonadotropic hormone on the acquisition of ovulatory competence in Japanese eel Anguilla japonica and bester sturgeon (Huso huso × Acipenser ruthenus). Indian Journal Science Technology. Proceedings of 9th International Sympo-sium on Reproductive Physiology of Fish, Cochin, India, 4(S8): 223–224.

Kalujnaia S, McWilliam IS, Zaguinaiko VA, Feilen AL, Nicholson J, Hazon N, Cutler CP, Balment RJ, Cossins AR, Hughes M, Cramb G. 2007. Salinity adaptation and gene profiling analysis in the European eel (Anguilla anguilla) using microarray technology. General and Comparative Endocrinology, 152(1): 274–280.

Kasuga Y. Adachi J, Nishi A, Hashimoto H, Kaji S, Horiuchi Y, Kagawa H. 2008. Induction of sexual maturation of male Japanese eel (Anguilla japonica) by continuous administration of various hormones using osmotic pump. Cybium, 32(2) suppl.: pg. 171.

Lokman PM, Wass RT, Sutter HC, Scott SG, Judge KF, Young G. 2001. Changes ste-roid hormone profiles and ovarian histology during salmon pituitary-induced vitellogenesis and ovulation in female New Zealand longfinned eels, Anguilla dieffenbachia Gray. Journal of Experimental Zoology. 289(1): 119–129.

Lubzens E, Cerda J. 2010. Oogenesis in teleost: how fish eggs are formed. General and Comparative Endocrinology, 10(3): 367–389.

Miura C, Miura T. 2011. Analysis of spermatogenesis using an eel model. Aqua-Bio-Science Monographs (ABSM), 4(4): 105–129.

Martínez-Porchas M, Martínez-Córdova LR, Ramos-Enriquez R. 2009. Cortisol and glucose: reliable indicators of fish stress? Pan-American Journal of Aquatic Science. 4(2): 158–178.

Nagahama Y, Yamashita M. 2008. Regulation of oocyte maturation in fish. Review. Development Growth Differentiation. 50 S: 159 – 218.

Patriche T. 2009. The importance of glucose determination in the blood of the cyprinids. Zootehnie si Biotehnologii, 42(2): 102–106.

Piferrer F, Blázquez. 2006. Aromatase distribution and regulation in fish. Review Fish Physiology and Biochemistry 31(2): 215–226.

Sudo R, Suetake H, Suzuki Y, Utoh T, Tanaka S, Aoyama J, Tsukamoto K. 2011. Dynamics of reproductive hormones during down-stream migration in females of the Japanese eel, Anguilla japonica. Zoological Science, 25(1): 180–188.

Suetake H, Okubo K, Yoshiura Y, Aida K. 2003. GTH and GnRH molecules and their expression in the Japanese eel. In: Aida K, Tsukamoto K, Yamauchi K. (Eds.). Eel Biology. Springer-Verlag Tokyo. pp. 351–372.

Tomkiewicz J, Kofoed TMN, Pedersen JS. 2011. Assessment of testis development during induced spermatogenesis in the European eel Anguilla anguilla marine and coastal fisheries: dynamics, management, and ecosystem. Science, 3(1): 106–118.

Tsai Ya-Ju, Lee Mong-Fong, Chen Chia-Yung, Chang Ching-Fong. 2011. Development of gonadal tissue and aromatase function in the protogynous orange-spotted grouper Epinephelus coioides. Zoological Studies, 50(6): 693–704.

Tubio RIC, Pérez-Maceira J, Aldegunde M. 2010. Homeostasis of glucose in the rainbow trout (Oncorhynchus mykiss Walbaum): the role of serotonin. The Journal of Experimental Biology. 213(1): 1813–1821.

Uno T, Ishizuka M, Itakura T. 2012. Cytochrome P450 (CYP) in Fish. Review. Environmental Toxicology and Pharmacology, 34(1): 1–13.

Van Ginneken V, Dufour S, Sbaihi M, Balm P, Noorlander K, de Bakker M, Doornbos J, Palstra A, Antonissen E, Mayer I, van den Thillart G. 2007. Does a 5500 km swim trial stimulate early sexual maturation in the European eel (Anguilla anguilla L.)?. Comparative Biochemistry and Physiology, Part A, 147: 1095–1103.

Van Ginneken V, Durif C, Dufour S, Sbaihi M, Boot R, Noorlander K, Doornbos J, Murk AJ, Van Den Thillart G. 2007a. Endocrine profiles during silvering of the European eel (Anguilla anguilla L.) living in salt-water. Animal Biology, 57(4): 453–465.

Van Ginneken V, Durif C, Balm SP, Boot R, Verstegen MWA, Antonissen E, Van den Thillart G. 2007b. Silvering of European eel (Anguilla anguilla): seasonal changes of morphological and methabolic parame-ters. Animal Biology, 57(1): 63–77.

Volkoff H, Unniappan S, Kelly SP. 2009. The endocrine regulation of food intake. In: Bernier NJ, Farrell AP, van der Krak G, Brauner CJ. (Eds.). Fish Physiology, 28: Fish Neuroendocrinology. Academic Press, London (UK). pp 421–465.

Weltzien F-A, Sébert M-E, Vidal B, Pasqualini C, Dufour S. 2009. Dopamine inhibition of eel reproduction. In: van den Thillart G, Dufour S, Rankin JC. (Eds.). Spawning migration of the European eel. Springer Science, London (UK). pp. 279–307.

Yaron Z, Levavi-Sivan B. 2011. Endocrine regulation of fish reproduction. Encyclopedia of fish physiology: From genome to envi-ronment, Vol. 2: 1500–1508.

Article Metrics

 10.32491/jii.v18i1.374
   Abstract views: 235   PDF views or download: 114

 

Copyright (c) 2018 Jurnal Iktiologi Indonesia
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Refbacks

  • There are currently no refbacks.