OPEN ACCESS PEER-REVIEWED | RESEARCH ARTICLE

Main Article Content

Authors

Irmawati Irmawati
Joeharnani Tresnati
Liestiaty Fachruddin
Nur Rahmawaty Arma
Andi Haerul

Abstract

The snakehead fish (Channidae) is widely distributed in inland water of Indonesia. This fish is native species in Suma-tera, Java and Kalimantan, but non-native species in Sulawesi and Papua. Study on molecular identification and phylo-geny of this fish using cytochrome c oxidase subunit I (COI) gene has only been conducted on snakehead fish origin from Tasikmalaya, Ambarawa, Bali, Aceh, Pontianak, and Banjarmasin waters, but none is available in South Sulawesi waters. The objectives of this research are to identify species of snakehead fish from Towuti Lake Sorowako and the first generation (F1) of domesticated snakehead fish from Bantaeng waters of South Sulawesi using COI gene for DNA barcoding, and to analyze the phylogenetic resolution of the fish. Partial sequences of the COI gene of the snakehead fish were aligned with sequences of snakehead fish deposited in GenBank. The phylogenetic tree was constructed using MEGA 7.0.20 program. The result indicated that COI gene nucleotides of snakehead fish from Towuti Lake Sorowako showed 99% homology with Channa striata acc no. KU692418 and KU692421, and showed 98% homology with those of acc no. KU852443. Therefore it can be conclude that Channa striata exist in Towuti Lake Sorowako. Nucleotide sequences of the first generation (F1) of domesticated snakehead fish from Bantaeng waters showed 65% homology with Channa pleurophthalma (acc no. KJ937390) origins from Banjarmasin waters and Channa gachua (acc no. KX389277). Based on this result, it assumed that snakehead fish from Towuti Lake Sorowako are distantly in gene to those from Bantaeng waters, and further analyses are required to identify the population of snakehead fish from Bantaeng waters.


Abstrak


 Populasi ikan gabus (famili Channidae) tersebar luas di wilayah perairan tawar Indonesia. Ikan gabus merupakan ikan asli di Sumatera, Jawa, dan Kalimantan) tetapi merupakan ikan introduksi di Sulawesi dan Papua. Identifikasi berdasar-kan gen cytochrome c oxidase subunit I (COI) telah dilakukan terhadap ikan gabus dari perairan di Tasikmalaya, Amba-rawa, Bali, Aceh, Pontianak dan Banjarmasin, tetapi ikan gabus dari perairan Sulawesi Selatan belum dilakukan. Tuju-an penelitian adalah untuk mengidentifikasi species ikan gabus dari Danau Towuti Sorowako dan ikan gabus generasi I (F1) hasil domestikasi dari induk yang berasal dari perairan tawar Kabupaten Bantaeng Sulawesi Selatan menggunakan gen COI untuk DNA barcoding, dan untuk menganalisis filogeni ikan gabus tersebut. Sekuen gen COI ikan gabus terse-but disejajarkan dengan sekuen nukleotida ikan gabus yang terdeposit di GenBank. Pohon filogenetik dikonstruksi de-ngan menggunakan program MEGA 7.0.20. Hasil penelitian menunjukkan bahwa nukleotida gen COI sampel ikan gabus dari Danau Towuti Sorowako memiliki kemiripan 99% dengan Channa striata nomor aksesi KU692418 dan KU692421 dan 98% dengan Channa striata nomor aksesi KU852443 sehingga dapat disimpulkan bahwa di Danau To-wuti terdapat ikan gabus jenis Channa striata. Tingkat kemiripan sekuen nukleotida ikan gabus F1dari perairan Kabu-paten Bantaeng adalah 65% dengan ikan gabus Channa pleurophthalma (KJ937390) asal perairan Banjarmasin dan ikan gabus Channa gachua (KX389277). Berdasarkan hasil tersebut maka diduga bahwa ikan gabus dari Danau Towu-ti Sorowako berkerabat jauh dengan ikan gabus dari perairan Bantaeng, dan diperlukan analisis yang lebih lanjut untuk menentukan jenis populasi ikan gabus F1 asal perairan tawar Kabupaten Bantaeng tersebut.

Keywords:
Channa striata; phylogenetic; cytochrome c oxidase subunit I (COI); snakehead fish;

Downloads article

Download data is not yet available.

Article Details

References

Arma NR, Illijas MA, Irmawati, Mappanyiwi A. 2014. Morphometric and meristic charac-teristics of snakehead fish for hatchery production. In: Hutauruk et al. (ed.). Proceeding of the 3rd International and National Seminar on Fisheries and Marine Science: Strengthening science and technology towards the development of Blue Economy, Faculty of Fisheries and Marine Science University of Riau, Riau. pp.198-201.

Dahruddin H, Hutama A, Busson F, Sauri S, Hanner R, Keith P, Hadiaty R, Hubert N. 2017. Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources, 17(2): 288-299.

Froese R, Pauly D. 2016. FishBase. World Wide Web electronic publication. www.fishbase. org version (10/2016).

Hebert PDN, Cywinska A, Ball SL, de Waard JR. 2003a. Biological identifications through DNA barcodes. In: Barrett S (ed.). Proceed-ings of The Royal Society B. Royal Society of London, 270(1512): 313-321.

Hebert PDN, Ratnasingham S, de Waard JR. 2003b. Barcoding animal life: cytochrome c oxidase subunit 1 divergences amongst closely related species. In: Barrett S (ed.). Proceedings of The Royal Society B. Royal Society of London, 270: S96-S99.

Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM. 2004. Identification birds through DNA barcodes. PloS Biology, 2(10): 1657-1663.

Herborg LM, Mandrak NE, Cudmore BC, Maclsaac HJ. 2007. Comparative distribution and invasion risk of snakehead (Channidae) and Asian carp (Cyprinidae) species in North America. Canadian Journal of Fish Aquatic Science, 64(12): 1723-1735.

Irmawati, Nadiarti, Tresnati J, Hidayani AA, Ar-ma NR. 2015. Reproductive performance of snakehead fish in tank. Disampaikan pada The 5th International Conference of Aquaculture Indonesia (ICAI): “Sustainable Aquaculture for the Future”, Jakarta 29-31 Oktober 2015.

Lambert DM, Baker A, Huynen L, Haddrath O, Hebert PDN, Millar CD. 2005. Is a large-scale DNA-based inventory of ancient life possible? Journal of Heredity, 96(3): 279–84.

Lakra WS, Goswami M, Gopalakrishnan A, Singh DP, Singh A, Nagpure NS. 2010. Genetic relatedness among fish species of genus Channa using mitochondrial DNA genes. Biochemical Systematics and Ecology, 38(6): 1212-1219.

Lynch M, Jarrell PE. 1993. A method for calibra-ting molecular clocks and its application to animal mitochondrial DNA. Genetics, 135(4): 1197-1208.

Ma H, Ma C, Ma L. 2012. Molecular identifica-tion of genus Scylla (Decapoda: Portunidae) based on DNA barcoding and polymerase chain reaction. Biochemical Systematics and Ecology, 41: 41-47.

Mat Jais AM, Dambisya YM, Lee TL. 1997. Antinociceptive activity of Channa striatus (haruan) extracts in mice. Journal of Ethno-pharmacology, 57(2): 125-130.

Michelle NYT, Shanti G, Loqman MY. 2004. Effect of orally administered Channa stria-tus extract against experimentally-induced osteoarthritis in rabbits. International Journal of Applied Research in Veterinary Me-dicine, 2(3):171-175.

Muchlisin ZA, Thomy Z, Fadli N, Sarong MA, Siti-azizah MN. 2013. DNA barcoding of freshwater fishes from Lake Laut Tawar, Aceh Province, Indonesia. Acta Ichthyologica et Piscatoria, 43(1): 21-29.

Mustafa, Widodo AMA, Kristianto Y. 2012. Albumin and zinc content of snakehead fish (Channa striatus) extract and is role and health. International Journal of Sciene and Technology, 1(2): 1-8.

Serrao NR, Steinke D, Hanner RH. 2014. Calibrating snakehead diversity with DNA Barcodes: expanding taxonomic coverage to enable identification of potential and established invasive species. Plos One, 9(6): 1-13.

Song LM, Munian K, Rashid AZ, Bhassu S. 2013. Characterisation of Asian snakehead Murrel Channa striata (Channidae) in Malaysia: An insight into molecular data and morphological approach. The scientific World Journal, 2013:1-16.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA 6: Molecular evolu-tionary genetics analysis version 6.0. Mole-cular Biology and Evolution, 30(12): 2725-2729.

Zhu SR, Ma KY, Xing ZJ, Xie N, Wang YX, Wang Q, Li JL. 2013. The complete mito-chondrial genome of Channa argus, Channa maculata and hybrid snakehead fish [Chan-na maculata (♀) x Channa argus (♂)]. Mi-tocondrial DNA, 24(3): 217-218.