OPEN ACCESS PEER-REVIEWED | RESEARCH ARTICLE

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Authors

Sheny Permatasari
Nur Bambang Priyo Utomo
Kukuh Nirmala

Abstract

This study aimed to evaluate the effect of the different doses of vitamin E in diet toward malondialdehide value and accumulation of heavy metal of Tilapia Oreochromis niloticus (Linnaeus 1758) exposed by lead (Pb). This study consists of four treatments, three replications and one negative control which was analyzed with descriptive method. Vitamin E supplementation treatments in the diet were KN (negative control 0 mg kg-1 , no exposed by lead); KP (positive control 0 mg kg-1 ); P100 (100 mg kg-1 ); P300 (300 mg kg-1 ); P500 (500 mg kg-1 ). Tilapia (13.17±1.06 g fish-1 ) were reared in 15 aquariums (135 liters volume) with a density of 13 fish in each aquarium for 60 days exposed by lead with 9.119 mg L-1 concentrations (except negative control). Fish were fed thrice a day at at 08.00 am, 12.00 pm, and 16.00 pm with the satiation level. An addition of Vitamin E alpha-tocopherol of 100 mg kg-1 in diet exposed by lead could decrease the accumulation of lead and MDA in fish liver of 1.47 mg kg-1 and 5.41 µg g-1, respectively.Abstrak Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan vitamin E dengan dosis berbeda pada pakan terha- dap nilai malondialdehid hati dan akumulasi logam berat pada tubuh ikan nila Oreochromis niloticus (Linnaeus, 1758) yang terpapar timbal (Pb). Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan empat perlaku- an dan tiga ulangan, dan satu perlakuan kontrol negatif yang diolah secara deskriptif. Perlakuan penambahan vitamin E: KN (kontrol negatif 0 mg kg-1 tanpa paparan Pb); KP (kontrol positif 0 mg kg-1 ); P100 (100 mg kg-1 ); P300 (300 mg kg-1 ); P500 (500 mg kg-1 ). Ikan nila (13,17±1,06 g ekor -1 ) dipelihara dalam 15 akuarium (volume 135 liter) dengan ke- padatan 13 ekor akuarium-1 selama 60 hari paparan Pb konsentrasi 9,119 mg L-1 kecuali kontrol negatif tanpa paparan. Ikan diberi pakan secara at satiation sebanyak tiga kali sehari pada pukul 08.00, 12.00, dan 16.00. Hasil penelitian me- nunjukkan bahwa vitamin E alpha-tokoperol sebanyak 100 mg kg-1 pada pakan ikan nila yang terpapar Pb (timbal) kon- sentrasi 9,119 mg L-1 dapat menurunkan nilai akumulasi logam Pb dan nilai malondialdehid hati ikan mencapai 1,47 mg kg-1 dan 5,41 µg g-1 .

Keywords:
accumulation of lead (Pb) , malondialdehid (MDA) , Oreochromis niloticus , Vitamin E (alpha-tocopherol)

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References

Clesceri LS, Greenberg AE, Trussell RR. 1989. Standard Method for the Examination of Water and Waste Water, APHA (America Public Health Association) 18th Edition, Washington DC.

Capeyron CJ, Eric B, Jean P, Pierre MR, Claude LL, Benard D . 2002. A diet cholesterol and deficient in vite incudes lipid peroxidation but does not enhace antioxidant enzyme expression in rat liver. The Journal of Nutritional Biochemistry, 13(5): 296-301.

El-Demerdash EM, Yousef MI, Kedwany FS, Baghdadhi HH. 2004. Cadmiun induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and b-carotene. Food and Chemical Toxicology, 42(10): 1563-1571.

El-Shebly AA. 2009. Protection of Nile tilapia (Oreochromis niloticus) from lead pollution and enhancement of its growth by α- tocopherol vitamin E. Research Journal of Fisheries and Hydrobiology. 4(1):17-21.

Harabawy ASA, Mosleh YTI. 2014. The role of vitamins A,C,E and selenium as antioxidants against genotoxicity and cytotoxicity of cadmium, copper, lead and zinc on erythrocytes of Nile tilapia, Oreochromis niloticus. Ecotoxicology and Environmental Safety, 104(1): 28-35.

Hashish EA, Elgaml SA, El-Murr A, Khalil R. 2015. Nephroprotective and antioxidant significance of selenium and alpha-tocopherol on lead acetate-induced toxicity of Nile tilapia (Oreochromis niloticus). Fish Physiology and Biochemistry, 41(3): 651- 660.

Henny C. 2011a.Kolong bekas tambang timah di Pulau Bangka: permasalahan kualitas air dan alternatif solusi untuk pemanfaatan. Oseanologi dan Limnologi Indonesia, 37(1): 119-138.

Henny C. 2011b. Bioakumulasi beberapa logam pada ikan di kolong bekas tambang timah di Pulau Bangka. Limnotek, 18(1):83-94.

Henny C, Susanti E. 2009. Karakteristik limnologis kolong bekas tambang timah di Pulau Bangka. Limnotek,16(2): 119-131.

Mahmoud MU, Ebied AM, Mohamed SM. 2013. Effect of lead on some haematological and biochemical characteristics of Clarias gariepinus dietary supplemented with lycopene and vitamin E. Egyptian Academic Journal of Biological Sciences, 5(1): 67- 89.

Sutari VT, Sugito, Aliza D, Asmarida. 2013. Ka- dar Malondialdehid (MDA) pada jaringan hati ikan nila (Oreochromis niloticus) yang diberi cekaman panas dan pakan suplementasi tepung daun jaloh (Salix tetrasperma Roxb). Jurnal Medika Veterania,7 (1): 35-38.

Schneider C. 2005. Chemistry and biology of vitamin E. Molecular Nutritionand Food Research, 49 (1):7-30.

Upasani CD, Khera A, Balaraman R. 2001. Ef- fect of lead with vitamin E, C or Spirulina on malondehyde, conjugated dienes and hydroperoxides in rats. Indian Journal of Experimental Biology, 9(1): 70-71.

Watanabe T. 1988. Fish Nutrition and Mariculture. Department of Aquatic Bioscience, Tokyo University of Fisheries. 233 p.

Yulaipi S, Aunurohim. 2013. Bioakumulasi logam berat timbal (Pb) dan hubungannya dengan laju pertumbuhan ikan mujair (Oreochromis mossambicus). Jurnal Sains dan Seni Pomits, 2(2): 166-170.